Q8YXD1 (LIPA2_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase 2
EC=2.8.1.8

Alternative name(s):
Lip-syn 2
Lipoate synthase 2
Lipoic acid synthase 2
Sulfur insertion protein lipA2

Gene names

Name:	lipA2
Ordered Locus Names:	alr1282

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00206.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Lipoyl synthase 2 HAMAP-Rule MF_00206		PRO_0000102283
Sites
	Metal binding	46	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	57	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	72	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	76	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	79	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YXD1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 26E05E9462CA7264	FASTA	300	33,491
	10 20 30 40 50 60 MTASQSAQFK SEITAMPSWL RRPIGKASEL STVQRIIKQR QIHTICEEGR CPNRGECYAQ 70 80 90 100 110 120 KTATFLLMGP TCTRVCAFCQ VDKGHAPMPL DPEEGQKVAE AVQLLGLRYV VLTSVARDDL 130 140 150 160 170 180 QDQGASHFVQ TMEAIRQLNP GTQIEVLTPD FWGGAGAGES GQRQRIKMIV EAQPACFNHN 190 200 210 220 230 240 IETVRRLTGP VRRGAKYDRS LRVLSLVKEL NPRIPTKSGL MVGYGETVAE LVEAMTDLRN 250 260 270 280 290 300 IGCDRLTIGQ YMRPSLEHLP VQKYWTPEEF EHLGKLAREM GFSHVRSAPL VRSSYHAGEE

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB73239.1.
PIR	AG1966.
RefSeq	NP_485325.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YXD1.
ModBase	Search...
Protein-protein interaction databases
STRING	103690.alr1282.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB73239; BAB73239; BAB73239.
GeneID	1104877.
KEGG	ana:alr1282.
PATRIC	22772518. VBINosSp37423_1743.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0320.
HOGENOM	HOG000235998.
KO	K03644.
OMA	PIGKASE.
ProtClustDB	PRK12928.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA2_NOSS1

Accession

Primary (citable) accession number: Q8YXD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program