Reviewed, UniProtKB/Swiss-Prot Q8YXA2 (LEU3_ANASP)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	alr1313

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	3-isopropylmalate dehydrogenase		PRO_0000083628
Regions
	Nucleotide binding	78 – 91	14	NAD By similarity
	Nucleotide binding	284 – 296	13	NAD By similarity
Sites
	Metal binding	226	1	Magnesium or manganese By similarity
	Metal binding	250	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Site	143	1	Important for catalysis By similarity
	Site	194	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YXA2-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E414E8BCC353E6D0	FASTA	362	38,769
	10 20 30 40 50 60 MTQNYRITLL PGDGIGPEIM AVAVDVLKVV GQQFDIQFDF QEALIGGAAI DATGEPLPSA 70 80 90 100 110 120 TLDTCRHSDA VLLAAIGGYK WDSLPPHQRP EGGLLGLRAG LELFANLRPA QILPQLIDAS 130 140 150 160 170 180 TLKREVVEGV DIMVVRELTG GIYFGKPRGI FTTETGEKRG VNTMVYTESE IDRIGRIAFE 190 200 210 220 230 240 TARKRRGKLC SVDKANVLDV SQLWRDRITN LSQEYPDVEL SHLYVDNAAM QLVRAPKQFD 250 260 270 280 290 300 TIVTGNLFGD ILSDAAAMLT GSIGMLPSAS LGASGPGVFE PVHGSAPDIA GQDKANPLAQ 310 320 330 340 350 360 VLSAAMMLRY ALDQPQAADK IEQAVLQVLE QGDRTGDIIS VGMNLLGCRG MGDSLIKALA KS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73270.1.
PIR	AF1970.
RefSeq	NP_485356.1.
3D structure databases
HSSP	HSSP built from PDB template 1A05 based on UniProtKB Q56268.
ModBase	Search...
Genome annotation databases
GeneID	1104908.
GenomeReviews	Gene locus alr1313 in contig BA000019_GR.
KEGG	ana:alr1313.
NMPDR	fig|103690.1.peg.1623.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YXA2.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1313-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_ANASP

Accession

Primary (citable) accession number: Q8YXA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	November 4, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)