Reviewed, UniProtKB/Swiss-Prot Q8YX58 (HEM62_ANASP)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic 2
      Short name=Coproporphyrinogenase 2
      Short name=Coprogen oxidase 2
    EC=1.3.3.3

Gene names

Name:	hemF2
Ordered Locus Names:	all1357

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Coproporphyrinogen-III oxidase, aerobic 2 HAMAP MF_00333		PRO_0000109881
Regions
	Region	75 – 84	10	Important for dimerization By similarity
	Region	135 – 137	3	Substrate binding By similarity
	Region	287 – 322	36	Important for dimerization By similarity
	Region	305 – 310	6	Substrate binding By similarity
Sites
	Active site	133	1	Proton donor By similarity
	Binding site	119	1	Substrate By similarity
	Site	197	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YX58-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 402D8DFB7BB51D29	FASTA	347	40,031
	10 20 30 40 50 60 MGRHSDNSLQ ESANHTVLLT SPTNTIPKDS RQRSQQFMQN LQDEICTALE QLDGKASFHQ 70 80 90 100 110 120 DHWERAEGGE GRTRVIREGR VFEQGGVNFS AVWGNSLPAS ILAQRPEAAG HEFFATGTSM 130 140 150 160 170 180 VLHPRNPYVP TVHLNYRYFE AGPIWWFGGG ADLTPYYAFK EDAVHFHQTL KNACDVHNPE 190 200 210 220 230 240 YYPTFKRWCD EYFYLRHREE QRGIGGIFFD YQDASGKLYV ESQTDSSVAI YSQKVGNVAR 250 260 270 280 290 300 NWEDIFAFVQ SCGQAFLPAY LPIVERRQGI EYGDRQRNFQ LYRRGRYVEF NLVYDRGTVF 310 320 330 340 GLQTKGRTES ILMSLPPLAR WEYCYEPEAG SPEAELTEVF LQPRDWV

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB73314.1.
PIR	AB1976.
RefSeq	NP_485400.1.
3D structure databases
SMR	Q8YX58. Positions 31-347.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YX58.
Genome annotation databases
GeneID	1104952.
GenomeReviews	Gene locus all1357 in contig BA000019_GR.
KEGG	ana:all1357.
NMPDR	fig|103690.1.peg.1667.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	GESTIIF.
PhylomeDB	Q8YX58.
Enzyme and pathway databases
BioCyc	NSP103690:ALL1357-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM62_ANASP

Accession

Primary (citable) accession number: Q8YX58

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)