Reviewed, UniProtKB/Swiss-Prot Q8YX57 (ACSF1_ANASP)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 1
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase 1
    EC=1.14.13.81

Gene names

Name:	acsF1
Ordered Locus Names:	alr1358

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 1		PRO_0000217526

Sequences

	Sequence		Length	Mass (Da)
	Q8YX57-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1740562764634453	FASTA	351	41,422
	10 20 30 40 50 60 MVNTLPKPGI KTPSKETVLT PRFYTTDFET AANLDLSAQE NELQAMLAEM RADYNRHHFV 70 80 90 100 110 120 RDEAFEQSWE HIDGEARQAF IEYLERSCIS EFSGFLLFKE LSRKLKNRSP VLAEMFQLMA 130 140 150 160 170 180 RDEARHAGFL NKAMGDFKLS LDLATVTKTR TYTFFPIEWV LYTVYLSEKI GYWRYIIIFR 190 200 210 220 230 240 HLEKHPENQF YPIFRYFESW CQDENRHGDI FKALLRSQPQ LWNNWKARLW SRFFLLSVFA 250 260 270 280 290 300 THTLTVHERS GFYKSLGLDA TDFDLQVVRN TNETAGRAFP VMLNTEHPKF FPLLQRCAGY 310 320 330 340 350 NLNISEIERS SQPKFVKLIR KLPLIAAIVW NLLMVYLIKP IDTEALRETV R

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73315.1.
PIR	AC1976.
RefSeq	NP_485401.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1104953.
GenomeReviews	Gene locus alr1358 in contig BA000019_GR.
KEGG	ana:alr1358.
NMPDR	fig|103690.1.peg.1668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YX57.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1358-MON.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF1_ANASP

Accession

Primary (citable) accession number: Q8YX57

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	March 1, 2002
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)