Reviewed, UniProtKB/Swiss-Prot Q8YX05 (SAHH_ANASP)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	alr1414

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116940
Regions
	Region	184 – 351	168	NAD binding By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YX05-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 38B232B91E0E46E2	FASTA	425	46,172
	10 20 30 40 50 60 MTATSPRLKH EVKDLALAPL GRQRIEWAGR EMPVLRQIRD RFAQEKPFAG LRLVACAHIT 70 80 90 100 110 120 TETAHLAIAL KAGGADAVLI ASNPLSTQDD VAASLVLDHE IPVFAQKGED NATYNRHVQI 130 140 150 160 170 180 ALDHRPNIII DDGSDVVATL VQERQHQIAD LIGTTEETTT GIVRLRAMFK DGVLTFPAVN 190 200 210 220 230 240 VNDADTKHFF DNRYGTGQST LDGIIRATNI LLAGKNVVVV GYGWCGKGTA LRARGMGANV 250 260 270 280 290 300 IVTEIDPIKA IEAVMDGFRV LPMAEAAPQG DIFITVTGNK HVVRGEHFDV MKDGAIVCNS 310 320 330 340 350 360 GHFDLELDLK YLAANAKEIK EVRPFTEEYK LTNGKSVVVL GQGRLINLAA AEGHPSAVMD 370 380 390 400 410 420 MSFANQALAC EYLVKNKGKL APGLHSIPVE VDQEIARLKL QAMGIYIDSL TPEQIEYINS WTSGT

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73371.1.
PIR	AC1983.
RefSeq	NP_485457.1.
3D structure databases
HSSP	HSSP built from PDB template 1K0U based on UniProtKB P10760.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YX05.
Genome annotation databases
GeneID	1105009.
GenomeReviews	Gene locus alr1414 in contig BA000019_GR.
KEGG	ana:alr1414.
NMPDR	fig|103690.1.peg.1724.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YX05.
OMA	IRLKAMA.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1414-MON.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_ANASP

Accession

Primary (citable) accession number: Q8YX05

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)