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Protein

Lipoyl synthase 1

Gene

lipA1

Organism
Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase 1 (lipA1), Lipoyl synthase 2 (lipA2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi39Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi45Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi60Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi64Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi67Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1UniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn 1UniRule annotation
Lipoate synthase 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Sulfur insertion protein lipA1
Gene namesi
Name:lipA1UniRule annotation
Ordered Locus Names:all1694
OrganismiNostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000002483 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022821 – 291Lipoyl synthase 1Add BLAST291

Interactioni

Protein-protein interaction databases

STRINGi103690.all1694

Structurei

3D structure databases

ProteinModelPortaliQ8YWC1
SMRiQ8YWC1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235997
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YWC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKPDWLRV KAPQWERVGN VKEILRDLAL NTVCEEASCP NIGECFQAGT
60 70 80 90 100
ATFLIMGPAC TRACPYCDID FEKKPKALDP TEPTRLAEAV RRMRLNHVVI
110 120 130 140 150
TSVNRDDLPD GGASQFVKCI AEVRAVSPQT TIEVLIPDLC GNWEALRIIL
160 170 180 190 200
QASPEVLNHN TETIPRLYRR VRPQGNYDRT LELLQRSRQI APWLYTKSGI
210 220 230 240 250
MVGLGETNAE VRQVMQDLRA VDCDILTIGQ YLQPSQKHLQ VNDFVTPEQF
260 270 280 290
AAWQVYGEEL GFLQVVSSPL TRSSYHAEQV RQLMERYPRN K
Length:291
Mass (Da):33,051
Last modified:March 1, 2002 - v1
Checksum:iED1F6297D0E07B34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA Translation: BAB78060.1
PIRiAH2017
RefSeqiWP_010995863.1, NC_003272.1

Genome annotation databases

EnsemblBacteriaiBAB78060; BAB78060; BAB78060
KEGGiana:all1694

Similar proteinsi

Entry informationi

Entry nameiLIPA1_NOSS1
AccessioniPrimary (citable) accession number: Q8YWC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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