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Q8YVX4 (TRMB_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:alr1845
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171285

Sites

Active site1251 By similarity
Binding site471S-adenosyl-L-methionine By similarity
Binding site721S-adenosyl-L-methionine By similarity
Binding site991S-adenosyl-L-methionine By similarity
Binding site1251S-adenosyl-L-methionine By similarity
Binding site1291Substrate By similarity
Binding site1611Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YVX4 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 6B255C82EA25C83C

FASTA21924,847
        10         20         30         40         50         60 
MISGVSALPF VRVRQHVNPL AQKYLTPANP LEWEKAYSTP HQPLHLDIGC ARGRFVLQMA 

        70         80         90        100        110        120 
QVEPNWNFLG LEIRESLVIE ANQFRSQLGL TNLHYLYCNA NNSLQPLLSS LPTGILQRVT 

       130        140        150        160        170        180 
IQFPDPWFKT RHAKRRVVQP ELVQDIANYL AVGGVVFLQS DMEFVAVEMC DRFAANPAFK 

       190        200        210 
KVGTGEWLTE NPLPVATERE TTTQNRGEPV YRALFERSS 

« Hide

References

[1]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000019 Genomic DNA. Translation: BAB73544.1.
PIRAG2036.
RefSeqNP_485885.1. NC_003272.1.

3D structure databases

ProteinModelPortalQ8YVX4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.alr1845.

Protocols and materials databases

DNASU1105437.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB73544; BAB73544; BAB73544.
GeneID1105437.
KEGGana:alr1845.
PATRIC22773769. VBINosSp37423_2361.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000237936.
KOK03439.
OMARVTIQFP.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_NOSS1
AccessionPrimary (citable) accession number: Q8YVX4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways