Reviewed, UniProtKB/Swiss-Prot Q8YVH1 (DEF2_ANASP)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 2
      Short name=PDF 2
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	all2007

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082731
Sites
	Active site	145	1	By similarity
	Metal binding	102	1	Iron By similarity
	Metal binding	144	1	Iron By similarity
	Metal binding	148	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YVH1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E17D0BC9B0E473D2	FASTA	179	20,136
	10 20 30 40 50 60 MNPELLPIIQ LGNPTLRQKA AWVENIHDAT IQQLIDDLIA TVAKANGVGI ASPQVAQSYR 70 80 90 100 110 120 LFIVASRPNP RYPHAPEMEP TAMINPKIVG HSTEIVEGWE GCLSVPGIRG LVPRHQAIEV 130 140 150 160 170 EYTDRYGNLQ KQTLTDFVAR IFQHEFDHLD GVLFIDRVES NLNTITEEEY QELVTKNTK

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73706.1.
PIR	AI2056.
RefSeq	NP_486047.1.
3D structure databases
HSSP	HSSP built from PDB template 1LME based on UniProtKB P96113.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YVH1.
Genome annotation databases
GeneID	1105602.
GenomeReviews	Gene locus all2007 in contig BA000019_GR.
KEGG	ana:all2007.
NMPDR	fig|103690.1.peg.2314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YVH1.
OMA	QRFTIWR.
Enzyme and pathway databases
BioCyc	NSP103690:ALL2007-MON.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF2_ANASP

Accession

Primary (citable) accession number: Q8YVH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)