Q8YVA8 (ARGJ1_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine biosynthesis bifunctional protein ArgJ 1

Cleaved into the following 2 chains:

1. Arginine biosynthesis bifunctional protein ArgJ1 alpha chain
2. Arginine biosynthesis bifunctional protein ArgJ1 beta chain

Including the following 2 domains:

1. Glutamate N-acetyltransferase
   EC=2.3.1.35
   Alternative name(s):
   Ornithine acetyltransferase
   Short name=OATase
   Ornithine transacetylase
2. Amino-acid acetyltransferase
   EC=2.3.1.1
   Alternative name(s):
   N-acetylglutamate synthase
   Short name=AGS

Gene names

Name:	argJ1
Ordered Locus Names:	alr2073

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm Probable HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the ArgJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Autocatalytic cleavage
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Arginine biosynthesis bifunctional protein ArgJ1 alpha chain By similarity		PRO_0000002097
	Chain	191 – 413	223	Arginine biosynthesis bifunctional protein ArgJ1 beta chain By similarity		PRO_0000002098
Sites
	Site	190 – 191	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YVA8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9268B3EA817D9134	FASTA	413	42,998
	10 20 30 40 50 60 MADWQEITGG ITAPKGYRAA GITAGLKPSG LPDLALIVSD VEAIASGVFT TSQVKAACVD 70 80 90 100 110 120 YCRQRLQAKQ SARAILCNAG QANAATGSQG IKDAEESAEL LAKELNISPE LILLASTGVI 130 140 150 160 170 180 GQRIKMDALR NGIPKLIASL TDTGSDAAAG AIITTDLVTK SIALETTIGD RPVRIGGIAK 190 200 210 220 230 240 GSGMIHPNMA TMLAFVTCDA AVSSHLWQQM LTRAADRSFN SITVDGDTST NDSLIALANG 250 260 270 280 290 300 QSRTPAITEV GAESEKLEAM LTAVCQHLAK AIARDGEGAT CLIEVQVTGA HDEQAARQIA 310 320 330 340 350 360 KTIAGSSLVK SAIFGRDPNW GRIAAAAGRA GVPFEQENLQ IQLGDFLLLD NGQPLPFDRA 370 380 390 400 410 AASAYLKQAA TGAYLQQDTV LISVKVGNGH GTGKAWGCDL SYDYVKINAE YTT

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB73772.1.
PIR	AC2065.
RefSeq	NP_486113.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YVA8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YVA8.
Protein family/group databases
MEROPS	T05.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1105668.
GenomeReviews	Gene locus alr2073 in contig BA000019_GR.
KEGG	ana:alr2073.
NMPDR	fig|103690.1.peg.2380.
PATRIC	22774270. VBINosSp37423_2606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1364.
HOGENOM	HBG284202.
OMA	GRDPNWG.
PhylomeDB	Q8YVA8.
ProtClustDB	PRK05388.
Enzyme and pathway databases
BioCyc	NSP103690:ALR2073-MONOMER.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
KO	K00620.
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. Arg_biosynth_ArgJ. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56266. Pept_S58_DmpA/Arg_biosyn_ArgJ. 1 hit.
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ1_NOSS1

Accession

Primary (citable) accession number: Q8YVA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program