Reviewed, UniProtKB/Swiss-Prot Q8YUM5 (ILVC_ANASP)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	all2315

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151268
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YUM5-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 3AF384B806CE752F	FASTA	331	36,011
	10 20 30 40 50 60 MARMYYDEDA NLDLLAGKTI AIIGYGSQGH AHALNLKDSG LNVIVGLYPG SKSAEKAQAA 70 80 90 100 110 120 GLTVKNVADA ANIADFIMIL LPDEVQKTVY KNEIEPNLQE GNTLAFAHGF NIHFGQVVPP 130 140 150 160 170 180 ANVDVVMVAP KGPGHLVRRT YEQGQGVPAL FAVYQDASGK ARDRALSYAK GIGGTRGGVL 190 200 210 220 230 240 ETTFREETET DLFGEQAVLC GGLSALIKAG FETLVEAGYQ PELAYFECLH EVKLIVDLVV 250 260 270 280 290 300 EGGLAKMRDS ISNTAEYGDY TRGPRIVNEQ TKAEMRKVLS EIQSGQFARE FVLENQSGKP 310 320 330 GFTAMRRQEA EHPIEEVGKD LRAMFSWLKK V

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB74014.1.
PIR	AD2095.
RefSeq	NP_486355.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	1105912.
GenomeReviews	Gene locus all2315 in contig BA000019_GR.
KEGG	ana:all2315.
NMPDR	fig|103690.1.peg.2622.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YUM5.
OMA	Q8YUM5. AHGFNIR.
Enzyme and pathway databases
BioCyc	NSP103690:ALL2315-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ANASP

Accession

Primary (citable) accession number: Q8YUM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)