Reviewed, UniProtKB/Swiss-Prot Q8YUB6 (SYD_ANASP)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	all2436

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00044
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000110817

Sequences

	Sequence		Length	Mass (Da)
	Q8YUB6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 361819D75ECEBF2F	FASTA	595	67,905
	10 20 30 40 50 60 MRTHYCGELR QKDIGETVTL YGWVDRRRDH GGVIFLDLRD RSGIVQVVSD PQRTPDSYEL 70 80 90 100 110 120 ANSLRNEYVV EITGRVTQRP EESLNTRIPT GEVEIYADKI ELLNGVRKQL PFQVSTADTE 130 140 150 160 170 180 TVREDLRLKY RYLDLRRDRM ARNIQLRHQV VKAMRRYLED VEGFIEVETP ILTRSTPEGA 190 200 210 220 230 240 RDYVLPSRVN PGEWFALPQS PQLFKQILMV SGLDRYYQIA RCFRDEDLRA DRQPEFTQLD 250 260 270 280 290 300 MEMSFMSEEE IIELNEKLVS YIFKTVKGVE LPLPFPRLTY AEAMERYGCD KPDTRYDLQL 310 320 330 340 350 360 VNVSDVMKDS GFKVFRDAVA NGGIVKILPI PNGNEQISNV RIKPGGDLFR EASEAGAKGL 370 380 390 400 410 420 AYIRVREDGE IDTIGAIKDN LSEEQKQEIL QRTGAKPGHL LLFGAGDAVT VNKTLDRLRQ 430 440 450 460 470 480 AIAKEFGLID PDKINLLWVV DFPMFEWNAD EKRLEALHHP FTAPHPDDLH DLKTARAQAY 490 500 510 520 530 540 DLVFNGFEVG GGSRRIYQRE VQEQVFETIG LSPEEAQNKF GFLLEAFEYG TPPHGGIAYG 550 560 570 580 590 LDRLVMLFAG EESIRDVIAF PKTQQARCLL TDAPSGVDVK QLKELHVAST YKPKS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB74135.1.
PIR	AE2110.
RefSeq	NP_486476.1.
3D structure databases
HSSP	HSSP built from PDB template 1L0W based on UniProtKB P36419.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YUB6.
Genome annotation databases
GeneID	1106033.
GenomeReviews	Gene locus all2436 in contig BA000019_GR.
KEGG	ana:all2436.
NMPDR	fig|103690.1.peg.2743.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YUB6.
OMA	VDRRRDH.
Enzyme and pathway databases
BioCyc	NSP103690:ALL2436-MON.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ANASP

Accession

Primary (citable) accession number: Q8YUB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)