Q8YU96 (ALR_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	alr2458

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.
Sequence caution	The sequence BAB74157.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Alanine racemase HAMAP MF_01201		PRO_0000114494
Sites
	Active site	48	1	Proton acceptor; specific for D-alanine By similarity
	Active site	278	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	48	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YU96 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 56F888B75D655BED	FASTA	386	42,160
	10 20 30 40 50 60 MASHQQCDTY AWFSQRAWVE IDLEALSHNV QQLKQFLSPR TQLMAVVKAD AYGHGAVTVA 70 80 90 100 110 120 QTALQAGASC LGVATVPEGI QLREAGIQAP ILILGATHTP EQIQAIAQWQ LQATIGSPKQ 130 140 150 160 170 180 ALIFSNTLET IQHDSPIPVH IKLDTGMSRL GTNWQQAGEF VQLVERLPHL DIASVYSHLA 190 200 210 220 230 240 TADSPDPGIM EEQHRRFEEA IAQIKTLGIK IPSLHLANSA ATLADPRLHY DMVRVGLAVY 250 260 270 280 290 300 GLYPAPHLQN KISLKPVIQV QARITHVKTI AAGTGVSYGH QFIAPHEMRL AVVSIGYADG 310 320 330 340 350 360 VPRNLSNKMQ VLIRGQRVPQ IGAITMDQLM IDASALPDLQ EGEIVTLLGK QGKEKITADD 370 380 WAEALNTISW EVLCGFKHRL PRVGVM

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB74157.1. Different initiation.
PIR	AC2113.
RefSeq	NP_486498.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YU96.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YU96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1106055.
GenomeReviews	Gene locus alr2458 in contig BA000019_GR.
KEGG	ana:alr2458.
NMPDR	fig|103690.1.peg.2765.
PATRIC	22775112. VBINosSp37423_3022.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	ARITHVK.
PhylomeDB	Q8YU96.
ProtClustDB	PRK00053.
Enzyme and pathway databases
BioCyc	NSP103690:ALR2458-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_NOSS1

Accession

Primary (citable) accession number: Q8YU96

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program