Q8YTE6 (ILVD_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	alr2771

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103425
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YTE6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0CDE28F7EEB7BD60	FASTA	563	59,130
	10 20 30 40 50 60 MSENFRSKAI TQGVQRSPNR AMLRAVGFQD ADFTKAIVGV ANGYSTITPC NMGINQLAQR 70 80 90 100 110 120 AEAGINTAGA KPQIFGTITI SDGISMGTEG MKYSLVSREV IADSIETVCN GQSLDGVIAI 130 140 150 160 170 180 GGCDKNMPGA MIAIARINIP AIFVYGGTIK PGHYNGKDLT VVSSFEAVGE YSAGKIDENE 190 200 210 220 230 240 LLAVERNACP GAGSCGGMYT ANTMSSAFEA LGMSLPYSST MAAEDDEKAD STEESAKVLV 250 260 270 280 290 300 EAIRHQLLPR QIITRKSIEN AISVIMAVGG STNAVLHFLA IARAAGVELN LDDFETIRGR 310 320 330 340 350 360 VPVLCDLKPS GRYVATDLHK AGGIPQVMKM LLVHGLLHGD CMTITGKTIA EVLADIPEEP 370 380 390 400 410 420 SPNQDVIRPW NNPMYAQGHL AILKGNLATE GAVAKITGVK NPVITGPAKV FESEEDCLDA 430 440 450 460 470 480 ILAGKIKAGD VIVIRYEGPK GGPGMREMLA PTSAIIGAGL GDSVGLITDG RFSGGTYGMV 490 500 510 520 530 540 VGHVAPEAAV GGAIALVQEG DSITIDAHAR SLQLNISDEE LTRRRANWQP RPPRYTTGIL 550 560 AKYAKLVASS SVGAVTDLDL FNK

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB74470.1.
PIR	AD2152.
RefSeq	NP_486811.1. NC_003272.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YTE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1106369.
GenomeReviews	Gene locus alr2771 in contig BA000019_GR.
KEGG	ana:alr2771.
NMPDR	fig|103690.1.peg.3078.
PATRIC	22775791. VBINosSp37423_3358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	Q8YTE6.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	NSP103690:ALR2771-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_NOSS1

Accession

Primary (citable) accession number: Q8YTE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program