Reviewed, UniProtKB/Swiss-Prot Q8YSZ3 (PANCY_ANASP)

Names and origin

Protein names

Recommended name:
    Bifunctional pantoate ligase/cytidylate kinase
Including the following 2 domains:
    1- Recommended name:
            Pantoate--beta-alanine ligase
              EC=6.3.2.1
        Alternative name(s):
            Pantothenate synthetase
            Pantoate-activating enzyme
    2- Recommended name:
            Cytidylate kinase
                Short name=CK
              EC=2.7.4.14
        Alternative name(s):
            Cytidine monophosphate kinase
              Short name=CMP kinase

Gene names

Name:	panC/cmk
Ordered Locus Names:	alr2936

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_01349 ATP + (d)CMP = ADP + (d)CDP. HAMAP MF_01349
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_01349
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the pantothenate synthetase family. In the C-terminal section; belongs to the cytidylate kinase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyrimidine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cytidylate kinase activity Inferred from electronic annotation. Source: HAMAP pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Bifunctional pantoate ligase/cytidylate kinase HAMAP MF_01349		PRO_0000239787
Regions
	Nucleotide binding	315 – 323	9	ATP By similarity
	Region	1 – 302	302	Pantoate--beta-alanine ligase HAMAP MF_01349
	Region	303 – 534	232	Cytidylate kinase HAMAP MF_01349

Sequences

	Sequence		Length	Mass (Da)
	Q8YSZ3-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 23434C86E3DFF1BC	FASTA	534	59,177
	10 20 30 40 50 60 MRLLTTVAAL RCYLNKRRWE SRLTASEEQI LDSMTSWYQT AIGLVPTMGS LHQGHLSLIE 70 80 90 100 110 120 RARHENSTVI VSIFINPLQF GPNEDYGRYP RTLEQDRQLC EQGGVDAIFA PSPEELGIPQ 130 140 150 160 170 180 KNIQESQVTQ VIPPSVMISG LCGHSRLGHF QGVATIVTKL LNLVQPDRAY FGQKDGQQLA 190 200 210 220 230 240 VIKRLVADLD LPVEIVACPT VREASGLACS SRNQYLTAQE KQQAAVLYRG LLQAEAAFKA 250 260 270 280 290 300 GVRYSSRLRE VVRQELAKVS SVLVEYIELV EPTTLMPLDK IQEEGMLAIA ARLGSTRLID 310 320 330 340 350 360 NTILRDRQPI IAIDGPAGAG KSTVARQVAT KLGLVYLDTG AMYRAVTWLV LQQGIAIDDD 370 380 390 400 410 420 CAIAELASKC KIELTPSQDL QSPVRVWIND TDVTQNIRTI EVTSQVSAIA AQAAVRQALV 430 440 450 460 470 480 KQQQRWGKRG GLVAEGRDIG THVFPDAEVK IFLTASVGER ARRRQQDFQK QGQPEVSLEQ 490 500 510 520 530 LEKDIAERDW KDSTRKVSPL QKAADAVELQ TDGLSISDVA SQIVDYYQQR LSQW

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB74635.1.
PIR	AI2172.
RefSeq	NP_486976.1.
3D structure databases
HSSP	HSSP built from PDB template 1N2E based on UniProtKB O06280.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YSZ3.
Genome annotation databases
GeneID	1106534.
GenomeReviews	Gene locus alr2936 in contig BA000019_GR.
KEGG	ana:alr2936.
NMPDR	fig|103690.1.peg.3243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YSZ3.
OMA	LGEKDWQ.
Enzyme and pathway databases
BioCyc	NSP103690:ALR2936-MON.
Family and domain databases
HAMAP	MF_01349. [Tree]
InterPro	IPR004821. Cyt_trans_rel. IPR003136. Cytidylate_kin. IPR011994. Cytidylate_kin_d. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02224. Cytidylate_kin. 1 hit. PF02569. Pantoate_ligase. 1 hit. [Graphical view]
ProDom	PD000657. Adenylate_kin. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00017. cmk. 1 hit. TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANCY_ANASP

Accession

Primary (citable) accession number: Q8YSZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)