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Protein

Bifunctional enzyme PyrF/PyrE

Gene

pyrFE

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).By similarity
Catalyzes the decarboxylation of orotidine monophosphate (OMP) to uridine monophosphate (UMP).By similarity

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 961Proton donorBy similarity
Binding sitei374 – 37415-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
Binding sitei375 – 37515-phosphoribose 1-diphosphateBy similarity
Binding sitei378 – 37815-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
Binding sitei380 – 38015-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity

GO - Molecular functioni

  1. orotate phosphoribosyltransferase activity Source: UniProtKB-EC
  2. orotidine-5'-phosphate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Glycosyltransferase, Lyase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
UPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme PyrF/PyrE
Including the following 2 domains:
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMP decarboxylase
Short name:
OMPDCase
Short name:
OMPdecase
Orotate phosphoribosyltransferase (EC:2.4.2.10)
Short name:
OPRT
Short name:
OPRTase
Gene namesi
Name:pyrFE
Ordered Locus Names:alr2945
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Bifunctional enzyme PyrF/PyrEPRO_0000134638Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi103690.alr2945.

Structurei

3D structure databases

ProteinModelPortaliQ8YSY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 273273OMP decarboxylaseAdd
BLAST
Regioni274 – 477204Orotate phosphoribosyltransferaseAdd
BLAST
Regioni400 – 40895-phosphoribose 1-diphosphate bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the OMP decarboxylase family. Type 2 subfamily.Curated
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000256439.
KOiK13421.
OMAiVKAHGTR.
OrthoDBiEOG6DZF33.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR013785. Aldolase_TIM.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
TIGR02127. pyrF_sub2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YSY4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIFFDKLHQN ISQNQSLLFV GLDPNPEMMP GRYSSQDIID GLWDWLQFII
60 70 80 90 100
AETADFVCAY KPTLGFYEAL GVPGLELLQK TLAAIPSHIP IILDAKHSDL
110 120 130 140 150
NTSTIFAKTV FTQWGVDAIT LSPYTGQDHV APFLVYPDKA VFILCSTSNP
160 170 180 190 200
GAEALQQYPT RESPLYLQVV QESKNWGTPE QLGLEVGTTN AEVLARIRQV
210 220 230 240 250
APERMIMVRS IWAEGGNLNR ILEVGLNTDG NGLLIPVPQD MLASANLSKE
260 270 280 290 300
IQSLRAEINQ VRDITVRDVA SCSVWLPDVF TVKQHPLHDL ILQLYDIDCI
310 320 330 340 350
MFGNFVQASG AVFPYYIDLR KIISNPQVFN QVLSGYENIV KNLTFDRLAG
360 370 380 390 400
IPYGSLPTAT GLALRLNCPM IFPRKEVKAH GTRRLIEGNF RTGEVVVVVD
410 420 430 440 450
DILISGKSVM EGADKLKSAG LNVHDIVVFI DHEQGVKDKL QANGYRGHAV
460 470
LTISEITSVL HQAGRINDEQ FLALTAE
Length:477
Mass (Da):52,826
Last modified:June 6, 2002 - v2
Checksum:i157D608B39BF23B4
GO

Sequence cautioni

The sequence BAB74644.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB74644.1. Different initiation.
PIRiAB2174.
RefSeqiNP_486985.1. NC_003272.1.
WP_010997096.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB74644; BAB74644; BAB74644.
GeneIDi1106543.
KEGGiana:alr2945.
PATRICi22776188. VBINosSp37423_3554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB74644.1. Different initiation.
PIRiAB2174.
RefSeqiNP_486985.1. NC_003272.1.
WP_010997096.1. NC_003272.1.

3D structure databases

ProteinModelPortaliQ8YSY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.alr2945.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB74644; BAB74644; BAB74644.
GeneIDi1106543.
KEGGiana:alr2945.
PATRICi22776188. VBINosSp37423_3554.

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000256439.
KOiK13421.
OMAiVKAHGTR.
OrthoDBiEOG6DZF33.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
UPA00070; UER00120.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR013785. Aldolase_TIM.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
TIGR02127. pyrF_sub2. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiPYRFE_NOSS1
AccessioniPrimary (citable) accession number: Q8YSY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: January 7, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.