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Q8YSY4

- PYRFE_NOSS1

UniProt

Q8YSY4 - PYRFE_NOSS1

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Protein
Bifunctional enzyme PyrF/PyrE
Gene
pyrFE, alr2945
Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.UniRule annotation
Catalyzes the decarboxylation of orotidine monophosphate (OMP) to uridine monophosphate (UMP) By similarity.UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 961Proton donor By similarity
Binding sitei374 – 37415-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding sitei375 – 37515-phosphoribose 1-diphosphate By similarity
Binding sitei378 – 37815-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding sitei380 – 38015-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

GO - Molecular functioni

  1. orotate phosphoribosyltransferase activity Source: UniProtKB-EC
  2. orotidine-5'-phosphate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Glycosyltransferase, Lyase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
UPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme PyrF/PyrE
Including the following 2 domains:
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMP decarboxylase
Short name:
OMPDCase
Short name:
OMPdecase
Orotate phosphoribosyltransferase (EC:2.4.2.10)
Short name:
OPRT
Short name:
OPRTase
Gene namesi
Name:pyrFE
Ordered Locus Names:alr2945
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Bifunctional enzyme PyrF/PyrEUniRule annotation
PRO_0000134638Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi103690.alr2945.

Structurei

3D structure databases

ProteinModelPortaliQ8YSY4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 273273OMP decarboxylaseUniRule annotation
Add
BLAST
Regioni274 – 477204Orotate phosphoribosyltransferaseUniRule annotation
Add
BLAST
Regioni400 – 40895-phosphoribose 1-diphosphate binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the OMP decarboxylase family. Type 2 subfamily.
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000256439.
KOiK13421.
OMAiVKAHGTR.
OrthoDBiEOG6DZF33.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR013785. Aldolase_TIM.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
TIGR02127. pyrF_sub2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YSY4-1 [UniParc]FASTAAdd to Basket

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MIFFDKLHQN ISQNQSLLFV GLDPNPEMMP GRYSSQDIID GLWDWLQFII    50
AETADFVCAY KPTLGFYEAL GVPGLELLQK TLAAIPSHIP IILDAKHSDL 100
NTSTIFAKTV FTQWGVDAIT LSPYTGQDHV APFLVYPDKA VFILCSTSNP 150
GAEALQQYPT RESPLYLQVV QESKNWGTPE QLGLEVGTTN AEVLARIRQV 200
APERMIMVRS IWAEGGNLNR ILEVGLNTDG NGLLIPVPQD MLASANLSKE 250
IQSLRAEINQ VRDITVRDVA SCSVWLPDVF TVKQHPLHDL ILQLYDIDCI 300
MFGNFVQASG AVFPYYIDLR KIISNPQVFN QVLSGYENIV KNLTFDRLAG 350
IPYGSLPTAT GLALRLNCPM IFPRKEVKAH GTRRLIEGNF RTGEVVVVVD 400
DILISGKSVM EGADKLKSAG LNVHDIVVFI DHEQGVKDKL QANGYRGHAV 450
LTISEITSVL HQAGRINDEQ FLALTAE 477
Length:477
Mass (Da):52,826
Last modified:June 6, 2002 - v2
Checksum:i157D608B39BF23B4
GO

Sequence cautioni

The sequence BAB74644.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000019 Genomic DNA. Translation: BAB74644.1. Different initiation.
PIRiAB2174.
RefSeqiNP_486985.1. NC_003272.1.
WP_010997096.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB74644; BAB74644; BAB74644.
GeneIDi1106543.
KEGGiana:alr2945.
PATRICi22776188. VBINosSp37423_3554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000019 Genomic DNA. Translation: BAB74644.1 . Different initiation.
PIRi AB2174.
RefSeqi NP_486985.1. NC_003272.1.
WP_010997096.1. NC_003272.1.

3D structure databases

ProteinModelPortali Q8YSY4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 103690.alr2945.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB74644 ; BAB74644 ; BAB74644 .
GeneIDi 1106543.
KEGGi ana:alr2945.
PATRICi 22776188. VBINosSp37423_3554.

Phylogenomic databases

eggNOGi COG0284.
HOGENOMi HOG000256439.
KOi K13421.
OMAi VKAHGTR.
OrthoDBi EOG6DZF33.

Enzyme and pathway databases

UniPathwayi UPA00070 ; UER00119 .
UPA00070 ; UER00120 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPi MF_01208. PyrE.
InterProi IPR013785. Aldolase_TIM.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
Pfami PF00215. OMPdecase. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SMARTi SM00934. OMPdecase. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR00336. pyrE. 1 hit.
TIGR02127. pyrF_sub2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiPYRFE_NOSS1
AccessioniPrimary (citable) accession number: Q8YSY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: September 3, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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