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Q8YSY4

- PYRFE_NOSS1

UniProt

Q8YSY4 - PYRFE_NOSS1

Protein

Bifunctional enzyme PyrF/PyrE

Gene

pyrFE

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (06 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).By similarity
    Catalyzes the decarboxylation of orotidine monophosphate (OMP) to uridine monophosphate (UMP).By similarity

    Catalytic activityi

    Orotidine 5'-phosphate = UMP + CO2.
    Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei96 – 961Proton donorBy similarity
    Binding sitei374 – 37415-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
    Binding sitei375 – 37515-phosphoribose 1-diphosphateBy similarity
    Binding sitei378 – 37815-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
    Binding sitei380 – 38015-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity

    GO - Molecular functioni

    1. orotate phosphoribosyltransferase activity Source: UniProtKB-EC
    2. orotidine-5'-phosphate decarboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
    2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Decarboxylase, Glycosyltransferase, Lyase, Transferase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    UniPathwayiUPA00070; UER00119.
    UPA00070; UER00120.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional enzyme PyrF/PyrE
    Including the following 2 domains:
    Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
    Alternative name(s):
    OMP decarboxylase
    Short name:
    OMPDCase
    Short name:
    OMPdecase
    Orotate phosphoribosyltransferase (EC:2.4.2.10)
    Short name:
    OPRT
    Short name:
    OPRTase
    Gene namesi
    Name:pyrFE
    Ordered Locus Names:alr2945
    OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
    Taxonomic identifieri103690 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000002483: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Bifunctional enzyme PyrF/PyrEPRO_0000134638Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi103690.alr2945.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YSY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 273273OMP decarboxylaseAdd
    BLAST
    Regioni274 – 477204Orotate phosphoribosyltransferaseAdd
    BLAST
    Regioni400 – 40895-phosphoribose 1-diphosphate bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the OMP decarboxylase family. Type 2 subfamily.Curated
    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0284.
    HOGENOMiHOG000256439.
    KOiK13421.
    OMAiVKAHGTR.
    OrthoDBiEOG6DZF33.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.40.50.2020. 1 hit.
    HAMAPiMF_01208. PyrE.
    InterProiIPR013785. Aldolase_TIM.
    IPR011995. OMPdecase_type-2.
    IPR001754. OMPdeCOase_dom.
    IPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PfamiPF00215. OMPdecase. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SMARTiSM00934. OMPdecase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR00336. pyrE. 1 hit.
    TIGR02127. pyrF_sub2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8YSY4-1 [UniParc]FASTAAdd to Basket

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    MIFFDKLHQN ISQNQSLLFV GLDPNPEMMP GRYSSQDIID GLWDWLQFII    50
    AETADFVCAY KPTLGFYEAL GVPGLELLQK TLAAIPSHIP IILDAKHSDL 100
    NTSTIFAKTV FTQWGVDAIT LSPYTGQDHV APFLVYPDKA VFILCSTSNP 150
    GAEALQQYPT RESPLYLQVV QESKNWGTPE QLGLEVGTTN AEVLARIRQV 200
    APERMIMVRS IWAEGGNLNR ILEVGLNTDG NGLLIPVPQD MLASANLSKE 250
    IQSLRAEINQ VRDITVRDVA SCSVWLPDVF TVKQHPLHDL ILQLYDIDCI 300
    MFGNFVQASG AVFPYYIDLR KIISNPQVFN QVLSGYENIV KNLTFDRLAG 350
    IPYGSLPTAT GLALRLNCPM IFPRKEVKAH GTRRLIEGNF RTGEVVVVVD 400
    DILISGKSVM EGADKLKSAG LNVHDIVVFI DHEQGVKDKL QANGYRGHAV 450
    LTISEITSVL HQAGRINDEQ FLALTAE 477
    Length:477
    Mass (Da):52,826
    Last modified:June 6, 2002 - v2
    Checksum:i157D608B39BF23B4
    GO

    Sequence cautioni

    The sequence BAB74644.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000019 Genomic DNA. Translation: BAB74644.1. Different initiation.
    PIRiAB2174.
    RefSeqiNP_486985.1. NC_003272.1.
    WP_010997096.1. NC_003272.1.

    Genome annotation databases

    EnsemblBacteriaiBAB74644; BAB74644; BAB74644.
    GeneIDi1106543.
    KEGGiana:alr2945.
    PATRICi22776188. VBINosSp37423_3554.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000019 Genomic DNA. Translation: BAB74644.1 . Different initiation.
    PIRi AB2174.
    RefSeqi NP_486985.1. NC_003272.1.
    WP_010997096.1. NC_003272.1.

    3D structure databases

    ProteinModelPortali Q8YSY4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 103690.alr2945.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB74644 ; BAB74644 ; BAB74644 .
    GeneIDi 1106543.
    KEGGi ana:alr2945.
    PATRICi 22776188. VBINosSp37423_3554.

    Phylogenomic databases

    eggNOGi COG0284.
    HOGENOMi HOG000256439.
    KOi K13421.
    OMAi VKAHGTR.
    OrthoDBi EOG6DZF33.

    Enzyme and pathway databases

    UniPathwayi UPA00070 ; UER00119 .
    UPA00070 ; UER00120 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.40.50.2020. 1 hit.
    HAMAPi MF_01208. PyrE.
    InterProi IPR013785. Aldolase_TIM.
    IPR011995. OMPdecase_type-2.
    IPR001754. OMPdeCOase_dom.
    IPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    Pfami PF00215. OMPdecase. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SMARTi SM00934. OMPdecase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR00336. pyrE. 1 hit.
    TIGR02127. pyrF_sub2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7120 / UTEX 2576.

    Entry informationi

    Entry nameiPYRFE_NOSS1
    AccessioniPrimary (citable) accession number: Q8YSY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 6, 2002
    Last sequence update: June 6, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3