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Q8YSY4 (PYRFE_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional enzyme PyrF/PyrE

Including the following 2 domains:

  1. Orotidine 5'-phosphate decarboxylase
    EC=4.1.1.23
    Alternative name(s):
    OMP decarboxylase
    Short name=OMPDCase
    Short name=OMPdecase
  2. Orotate phosphoribosyltransferase
    Short name=OPRT
    Short name=OPRTase
    EC=2.4.2.10
Gene names
Name:pyrFE
Ordered Locus Names:alr2945
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP-Rule MF_01208

Catalyzes the decarboxylation of orotidine monophosphate (OMP) to uridine monophosphate (UMP) By similarity. HAMAP-Rule MF_01208

Catalytic activity

Orotidine 5'-phosphate = UMP + CO2. HAMAP-Rule MF_01208

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_01208

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP-Rule MF_01208

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.

Sequence similarities

In the N-terminal section; belongs to the OMP decarboxylase family. Type 2 subfamily.

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Sequence caution

The sequence BAB74644.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Bifunctional enzyme PyrF/PyrE HAMAP-Rule MF_01208
PRO_0000134638

Regions

Region1 – 273273OMP decarboxylase HAMAP-Rule MF_01208
Region274 – 477204Orotate phosphoribosyltransferase HAMAP-Rule MF_01208
Region400 – 40895-phosphoribose 1-diphosphate binding By similarity

Sites

Active site961Proton donor By similarity
Binding site37415-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site37515-phosphoribose 1-diphosphate By similarity
Binding site37815-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site38015-phosphoribose 1-diphosphate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YSY4 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: 157D608B39BF23B4

FASTA47752,826
        10         20         30         40         50         60 
MIFFDKLHQN ISQNQSLLFV GLDPNPEMMP GRYSSQDIID GLWDWLQFII AETADFVCAY 

        70         80         90        100        110        120 
KPTLGFYEAL GVPGLELLQK TLAAIPSHIP IILDAKHSDL NTSTIFAKTV FTQWGVDAIT 

       130        140        150        160        170        180 
LSPYTGQDHV APFLVYPDKA VFILCSTSNP GAEALQQYPT RESPLYLQVV QESKNWGTPE 

       190        200        210        220        230        240 
QLGLEVGTTN AEVLARIRQV APERMIMVRS IWAEGGNLNR ILEVGLNTDG NGLLIPVPQD 

       250        260        270        280        290        300 
MLASANLSKE IQSLRAEINQ VRDITVRDVA SCSVWLPDVF TVKQHPLHDL ILQLYDIDCI 

       310        320        330        340        350        360 
MFGNFVQASG AVFPYYIDLR KIISNPQVFN QVLSGYENIV KNLTFDRLAG IPYGSLPTAT 

       370        380        390        400        410        420 
GLALRLNCPM IFPRKEVKAH GTRRLIEGNF RTGEVVVVVD DILISGKSVM EGADKLKSAG 

       430        440        450        460        470 
LNVHDIVVFI DHEQGVKDKL QANGYRGHAV LTISEITSVL HQAGRINDEQ FLALTAE 

« Hide

References

[1]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000019 Genomic DNA. Translation: BAB74644.1. Different initiation.
PIRAB2174.
RefSeqNP_486985.1. NC_003272.1.

3D structure databases

ProteinModelPortalQ8YSY4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.alr2945.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB74644; BAB74644; BAB74644.
GeneID1106543.
KEGGana:alr2945.
PATRIC22776188. VBINosSp37423_3554.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0284.
HOGENOMHOG000256439.
KOK13421.
OMAVKAHGTR.
OrthoDBEOG6DZF33.

Enzyme and pathway databases

UniPathwayUPA00070; UER00119.
UPA00070; UER00120.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
3.40.50.2020. 1 hit.
HAMAPMF_01208. PyrE.
InterProIPR013785. Aldolase_TIM.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00215. OMPdecase. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
SMARTSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR00336. pyrE. 1 hit.
TIGR02127. pyrF_sub2. 1 hit.
ProtoNetSearch...

Entry information

Entry namePYRFE_NOSS1
AccessionPrimary (citable) accession number: Q8YSY4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: June 11, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways