Q8YSL0 (LPXD_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	alr3074

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059642
Sites
	Active site	246	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YSL0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 948A29A274EC6C2B	FASTA	349	37,431
	10 20 30 40 50 60 MKFSDIISKL EVTNHSLTQN PNNDPEITGM AAIDEATSAN LSYIEGAKFA SFIAQTNASA 70 80 90 100 110 120 LILPQNEAMQ AQAQARGIVW MTTPEPRLLF AKAIALFYQP YTPTPEIHPT AVIHPTAKIG 130 140 150 160 170 180 NDVYIGPHVV IQPGVEIGNG VIIHPNVVIY PYVKIGDRSI LHANCTIEER SQIGADCIIH 190 200 210 220 230 240 SGAVIGGEGF GFVPTRTGWY KMEQSGYVVL EDRVDIGCNT TIDRPSVGET RVGYDTKIDN 250 260 270 280 290 300 LVQIAHGCQI GAGCAIAAQT GMAGGVKLGK RVILAGQVGI ANQAKMGDGS TASAQTGILH 310 320 330 340 DVKPGEVVSG TPAIPHKIYL KIGAIYSRLP EMYQAFRQLQ RQSDKESKR

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB74773.1.
PIR	AC2190.
RefSeq	NP_487114.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YSL0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YSL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1106672.
GenomeReviews	Gene locus alr3074 in contig BA000019_GR.
KEGG	ana:alr3074.
NMPDR	fig|103690.1.peg.3381.
PATRIC	22776484. VBINosSp37423_3702.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	AKISTLF.
PhylomeDB	Q8YSL0.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	NSP103690:ALR3074-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_NOSS1

Accession

Primary (citable) accession number: Q8YSL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program