Reviewed, UniProtKB/Swiss-Prot Q8YSI2 (PROB_ANASP)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	alr3103

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109629
Regions
	Domain	286 – 364	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q8YSI2-1 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: 1522431CFC97E712	FASTA	378	40,794
	10 20 30 40 50 60 MNFELERSDL TKTIVVKIGT SSLTQPETGQ LALSTIATLA ETLSHLRQQG NRVILVSSGA 70 80 90 100 110 120 VGVGCARLGL TERPKAIALK QAVAAVGQGR LMRVYDDLFT TLQQPIAQVL LTRSDLVQRS 130 140 150 160 170 180 RYLNVYNTFR ELLALGVIPV VNENDTVAVD ELKFGDNDTL SALVASLVEA DWLFLLTDVD 190 200 210 220 230 240 RLYSADPRSV PDARPISLVT SIKELADLQV QTGSQGSQWG TGGMMTKISA ARIAIAAGVR 250 260 270 280 290 300 TVITQGRYPR NIEKIIQGEL IGTHFQPQPE PTSARKRWIA YGLVPVGKLY LDSGAIAAIV 310 320 330 340 350 360 KAGKSLLPAG VKTVAGEFEP QDAVQLCDPQ GNEIARGLVN YSSNDLQKIC GRHSKEIPTI 370 LGYVGAETVI HRDNLVLT

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB74802.1. Different initiation.
PIR	AH2193.
RefSeq	NP_487143.2.
3D structure databases
SMR	Q8YSI2. Positions 11-378.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YSI2.
Genome annotation databases
GeneID	1106701.
GenomeReviews	Gene locus alr3103 in contig BA000019_GR.
KEGG	ana:alr3103.
NMPDR	fig|103690.1.peg.3410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TDVDRLY.
PhylomeDB	Q8YSI2.
Enzyme and pathway databases
BioCyc	NSP103690:ALR3103-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ANASP

Accession

Primary (citable) accession number: Q8YSI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	July 26, 2002
Last modified:	February 9, 2010
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)