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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei637 – 6371ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:alr3283
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 872872Leucine--tRNA ligasePRO_0000151961Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi103690.alr3283.

Structurei

3D structure databases

ProteinModelPortaliQ8YS09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi634 – 6385"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YS09-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSRYNPATL EEKWQKTWVE LGLDKTQTQS NKPKFYALSM FPYPSGSLHM
60 70 80 90 100
GHVRNYTITD VIARLKRMQG YRVLHPMGWD AFGLPAENAA IDRGVPPAKW
110 120 130 140 150
TYQNITQMRQ QLQRLGLSID WDSEVATCSP DYYKWTQWIF LQFLQAGLAY
160 170 180 190 200
QKEAAVNWDP IDQTVLANEQ VDNEGRSWRS GAIVERKLLR QWFLKITDYA
210 220 230 240 250
EELLNDLDKL TGWPERVKLM QANWIGKSSG AYLEFPIVGS NEKIAVYTTR
260 270 280 290 300
PDTVYGVSYV VLAPEHPLTK QVTTKTQQAA VDTFIQEVTN QSELERTAED
310 320 330 340 350
KPKRGIATGG KAINPFTGEE VPIWIADYVL YEYGTGAVMG VPAHDVRDFK
360 370 380 390 400
FAQRYDLPID FVIAAPDDVA GFDLSPTSET EEVTQVVQIE YNQAYTEPGI
410 420 430 440 450
LINSGAFTGM TSTDAKQAIV KYATEKGFGK ERIQYRLRDW LISRQRYWGA
460 470 480 490 500
PIPVIHCPNC GIVPVPDKDL PVILPEEVEF TGRGGSPLAQ LESWVNVPCP
510 520 530 540 550
TCGTPAKRET DTMDTFIDSS WYFLRFTDAR NEAQVFESAK TNDWMPVDQY
560 570 580 590 600
VGGIEHAILH LLYSRFFTKV LRDRGLLNFD EPFERLLTQG MVQGLTYFNP
610 620 630 640 650
NKGGKDKWVP SHLVNPNDPR DPQTGEPLQR LYATMSKSKG NGVAPEDVIA
660 670 680 690 700
KYGVDTARMF ILFKAPPEKD LEWDEADVEG QFRFLNRVWR LVTDYVASGV
710 720 730 740 750
NPKNKSGELS KSEKDLRRAI HTAIQSVTED LEDDYQFNTA ISELMKLSNA
760 770 780 790 800
LTDANGKDSR VYAEGIKTLV VLLAPFAPHI AEELWRLLGN SESVHTQTWP
810 820 830 840 850
AFDPAALVAD EITLVIQVNG KKRADIQVPS QADKAELEKY ARESEVVQRH
860 870
LEGKEIKKVI VVPGKLVNFV VG
Length:872
Mass (Da):98,549
Last modified:March 1, 2002 - v1
Checksum:iA7B4CAD72C643C0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB74982.1.
PIRiAD2216.
RefSeqiNP_487323.1. NC_003272.1.
WP_010997434.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB74982; BAB74982; BAB74982.
GeneIDi1106881.
KEGGiana:alr3283.
PATRICi22776940. VBINosSp37423_3928.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB74982.1.
PIRiAD2216.
RefSeqiNP_487323.1. NC_003272.1.
WP_010997434.1. NC_003272.1.

3D structure databases

ProteinModelPortaliQ8YS09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.alr3283.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB74982; BAB74982; BAB74982.
GeneIDi1106881.
KEGGiana:alr3283.
PATRICi22776940. VBINosSp37423_3928.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiSYL_NOSS1
AccessioniPrimary (citable) accession number: Q8YS09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.