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Q8YRD8 (PUR2_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:alr3510
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151433

Regions

Domain109 – 315207ATP-grasp
Nucleotide binding135 – 19561ATP By similarity

Sites

Metal binding2851Magnesium or manganese By similarity
Metal binding2871Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YRD8 [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: 4FE53C13BB06CC1B

FASTA42544,390
        10         20         30         40         50         60 
MKVLVVGNGG REHALAWKLL QSPQIEQVAC VPGNGGTATL ARCQNVSLAV DDFEGISQYA 

        70         80         90        100        110        120 
LNQGFSLVVV GPEVPLAKGI TDYLQEKGLM VFGPSRAGAQ IEASKAWAKA LMQEAGIPTA 

       130        140        150        160        170        180 
KAAVFTEAAA AKSYIQAQGA PIVVKADGLA AGKGVTVATT IEQAQSAIDA IFQGQFGSAG 

       190        200        210        220        230        240 
EFVVIEECLT GQEVSVLALT DGLTIRPLLP AQDHKRIGEG DTGENTGGMG AYAPAPIATP 

       250        260        270        280        290        300 
ELMALVQTEV LERAIATLQS KGIDYRGVLY AGLMITPNGD FKVLEFNCRF GDPETQVILP 

       310        320        330        340        350        360 
LLATPLEELI LACVQQRLGE LPPIAWQGGA SATVVAASGG YPGDYEKGQV ITGIPQAEAT 

       370        380        390        400        410        420 
GATVFHAGTK LNAQQQIVTD GGRVLNVTGI GENFQQALAQ AYTGIKAIDF SGIYYRRDIG 


YRVNS 

« Hide

References

[1]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000019 Genomic DNA. Translation: BAB75209.1.
PIRAG2244.
RefSeqNP_487550.1. NC_003272.1.

3D structure databases

ProteinModelPortalQ8YRD8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.alr3510.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB75209; BAB75209; BAB75209.
GeneID1107108.
KEGGana:alr3510.
PATRIC22777426. VBINosSp37423_4169.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_NOSS1
AccessionPrimary (citable) accession number: Q8YRD8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways