Reviewed, UniProtKB/Swiss-Prot Q8YRD8 (PUR2_ANASP)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	alr3510

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151433
Regions
	Domain	109 – 315	207	ATP-grasp
	Nucleotide binding	135 – 195	61	ATP By similarity
Sites
	Metal binding	285	1	Magnesium or manganese By similarity
	Metal binding	287	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YRD8-1 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: 4FE53C13BB06CC1B	FASTA	425	44,390
	10 20 30 40 50 60 MKVLVVGNGG REHALAWKLL QSPQIEQVAC VPGNGGTATL ARCQNVSLAV DDFEGISQYA 70 80 90 100 110 120 LNQGFSLVVV GPEVPLAKGI TDYLQEKGLM VFGPSRAGAQ IEASKAWAKA LMQEAGIPTA 130 140 150 160 170 180 KAAVFTEAAA AKSYIQAQGA PIVVKADGLA AGKGVTVATT IEQAQSAIDA IFQGQFGSAG 190 200 210 220 230 240 EFVVIEECLT GQEVSVLALT DGLTIRPLLP AQDHKRIGEG DTGENTGGMG AYAPAPIATP 250 260 270 280 290 300 ELMALVQTEV LERAIATLQS KGIDYRGVLY AGLMITPNGD FKVLEFNCRF GDPETQVILP 310 320 330 340 350 360 LLATPLEELI LACVQQRLGE LPPIAWQGGA SATVVAASGG YPGDYEKGQV ITGIPQAEAT 370 380 390 400 410 420 GATVFHAGTK LNAQQQIVTD GGRVLNVTGI GENFQQALAQ AYTGIKAIDF SGIYYRRDIG YRVNS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB75209.1.
PIR	AG2244.
RefSeq	NP_487550.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YRD8.
Genome annotation databases
GeneID	1107108.
GenomeReviews	Gene locus alr3510 in contig BA000019_GR.
KEGG	ana:alr3510.
NMPDR	fig|103690.1.peg.3817.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YRD8.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	NSP103690:ALR3510-MON.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_ANASP

Accession

Primary (citable) accession number: Q8YRD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)