Q8YR06 (PURL_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	all3652

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	782 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 782	782	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100430
Regions
	Nucleotide binding	105 – 116	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YR06 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: CBFEBD6850DBC40A	FASTA	782	83,581
	10 20 30 40 50 60 MTATSPFSPQ EIAAEGIKPE EYAEIVRRLG RHPNKAELGM FGVMWSEHCC YKNSRPLLKQ 70 80 90 100 110 120 FPTTGPRILV GPGENAGVVD LGEGLQLAFK IESHNHPSAV EPFQGAATGV GGILRDIFTM 130 140 150 160 170 180 GARPIALLNS LRFGSLEDPK TQRLFSGVVA GISHYGNCVG VPTVGGEVYF DPAYSGNPLV 190 200 210 220 230 240 NVMALGLMET PEIVKSGAAG IGNPVLYVGS TTGRDGMGGA SFASAELSDE SIDDRPAVQV 250 260 270 280 290 300 GDPFLEKSLI EACLEAFKTG AVVAAQDMGA AGITCSTSEM AAKGGVGIEL DLDKIPVRET 310 320 330 340 350 360 RMVPYEYLLS ESQERMLFVA HQGREQELID IFHRWGLQAV VAGTVIAEPI VRIFFQGAIA 370 380 390 400 410 420 AEIPADALAE NTPLYERELL AEPPEYARLA WEWSSEALPT GTTAGIEIQG NLHSWQDILL 430 440 450 460 470 480 TLLNTPTIAS KNWVYRQYDH QVQNNTVFLP GGADAAVVRL RPLEGQGSRG AGEQGGVNSL 490 500 510 520 530 540 SGVAATVDCN PRYVYLDPYE GAKAVVAEAA RNLSCVGAEP LAVTDNLNFG SPEKPIGYWQ 550 560 570 580 590 600 LSEACRGLAE GCRELATPVT GGNVSLYNET FDTQGNPQPI YPTPVVGMVG LIADLTKICG 610 620 630 640 650 660 QGWQGVGDVI YLLGASITTL GASEYLATIH NTVAGRPPRV DFDLERRVQK VCREGIHAGS 670 680 690 700 710 720 VRSAHDCAEG GLAVALAESC LAGNLGAEIH LEVSPTQLQR LDEVLFGEGG ARILVSVVST 730 740 750 760 770 780 QQATWESYLQ EHLGQNWQKL GIVGNTDTDL AVLTTDNQTL IRVSIEEMND RYQNAIARRL AL

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB75351.1.
PIR	AE2262.
RefSeq	NP_487692.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YR06.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YR06.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1107250.
GenomeReviews	Gene locus all3652 in contig BA000019_GR.
KEGG	ana:all3652.
NMPDR	fig|103690.1.peg.3959.
PATRIC	22777758. VBINosSp37423_4333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q8YR06.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	NSP103690:ALL3652-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_NOSS1

Accession

Primary (citable) accession number: Q8YR06

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program