Reviewed, UniProtKB/Swiss-Prot Q8YQ59 (SYS_ANASP)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	serS
Ordered Locus Names:	all3976

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. HAMAP MF_00176
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity. HAMAP MF_00176
Subcellular location	Cytoplasm By similarity HAMAP MF_00176.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. HAMAP MF_00176
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Seryl-tRNA synthetase HAMAP MF_00176		PRO_0000121994
Regions
	Nucleotide binding	266 – 268	3	ATP By similarity
	Nucleotide binding	353 – 356	4	ATP By similarity
	Region	235 – 237	3	Serine binding By similarity
Sites
	Binding site	289	1	Serine By similarity
	Binding site	389	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YQ59-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E3EB9E94894E9DBB	FASTA	426	47,676
	10 20 30 40 50 60 MLDIKQIREN PQLIQERLNS RNGTYDIQPI LQLDKQQREL EATRSQIQAR SNEIGKIVGQ 70 80 90 100 110 120 KIKSGINPQD PEIQALRDEG NAIKAQLSEL EPREKELKAE IEQLILALPN LPSDSTPIGK 130 140 150 160 170 180 GEEENVEVRL WGDEYLPQNP NIIPHWEIGE KLGILNFERA VKVAQSRFVN LIGAGAALER 190 200 210 220 230 240 ALINFMLKMQ TAAGYIEVSP PLLVNTDSLT GTGQLPKFAE ESFKCADDEL WLIPTAEVPV 250 260 270 280 290 300 TNLYRGEILA AENLPIYHCA YTPCFRREAG SYGRDMRGLI RLHQFNKVEL VKVVHPSTSF 310 320 330 340 350 360 DELEKLVGNA EAILQALKLP YRVINLCTGD LGFGATKTYD LEVWLPSSGK YREISSCSNC 370 380 390 400 410 420 FDFQARRADI RFKEAGKKGT QFVHTLNGSG LAVGRTMAAI LENYQQPDGT ILIPEVLQPF LGREVL

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB75675.1.
PIR	AI2302.
RefSeq	NP_488016.1.
3D structure databases
SMR	Q8YQ59. Positions 1-426.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YQ59.
Genome annotation databases
GeneID	1107574.
GenomeReviews	Gene locus all3976 in contig BA000019_GR.
KEGG	ana:all3976.
NMPDR	fig|103690.1.peg.4283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0172.
HOGENOM	HBG629391.
OMA	WSSCYRR.
PhylomeDB	Q8YQ59.
Enzyme and pathway databases
BioCyc	NSP103690:ALL3976-MONOMER.
Family and domain databases
HAMAP	MF_00176. Ser_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. IPR010978. tRNA_bd_arm. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_ANASP

Accession

Primary (citable) accession number: Q8YQ59

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)