Reviewed, UniProtKB/Swiss-Prot Q8YPS9 (MURD_ANASP)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	alr4112

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108955
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YPS9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C5E8EE008A5CD7D6	FASTA	462	50,314
	10 20 30 40 50 60 MSKAHVVGLG KSGVAAARLL KREGWEVVLS DSNTSDTLLK QQQELAKEQI TVELGYSLDF 70 80 90 100 110 120 AGALPDLIIV SPGVPWDIPD LVKARDLGIE TIGEMELAWR HLKSLPWVGI TGTNGKTTTT 130 140 150 160 170 180 ALIAAIFQAA GFDAPACGNI GYAACEVALA EIPPDWIIGE MSSYQIESSV TLAPHISIWT 190 200 210 220 230 240 TFTPDHLARH KTLENYYDIK AKLLRQSHLQ VFNGDDAYLS KIGASHWPDA YWTSVQGKES 250 260 270 280 290 300 LLGEKGFYIE DGWVVEQLPN SPPQRIVEAS ALRMVGAHNL QNLLMAVAAA RLADISPNAI 310 320 330 340 350 360 DKAVREFPGV AHRLEHICTW EGIDFINDSK ATNYDAAEVG LASVKSPVVL IAGGEAKPGD 370 380 390 400 410 420 DTAWLAKIQA QTSAVLLIGS AAPAFAQRLK EVGYTHYEIV ETMEKAVRRS LELAKHHQAP 430 440 450 460 VVLLSPACAS FDQYPNFEAR GDHFRELCLE LVGEKETNSN HH

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB75811.1.
PIR	AI2319.
RefSeq	NP_488152.1.
3D structure databases
HSSP	HSSP built from PDB template 1EEH based on UniProtKB P14900.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YPS9.
Genome annotation databases
GeneID	1107714.
GenomeReviews	Gene locus alr4112 in contig BA000019_GR.
KEGG	ana:alr4112.
NMPDR	fig|103690.1.peg.4419.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YPS9.
OMA	LWVNDTK.
Enzyme and pathway databases
BioCyc	NSP103690:ALR4112-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_ANASP

Accession

Primary (citable) accession number: Q8YPS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)