Q8YPR1 (PGK_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	all4131

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity. HAMAP MF_00145
Subcellular location	Cytoplasm Potential HAMAP MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.
Mass spectrometry	Molecular mass is 42472 Da from positions 1 - 400. Determined by MALDI. Ref.2

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Phosphoglycerate kinase HAMAP MF_00145		PRO_0000145897
Regions
	Nucleotide binding	356 – 359	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	63 – 66	4	Substrate binding By similarity
Sites
	Binding site	40	1	Substrate By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	205	1	ATP By similarity
	Binding site	296	1	ATP; via carbonyl oxygen By similarity
	Binding site	327	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YPR1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: FA9EBE9015D4C0F9	FASTA	400	42,441
	10 20 30 40 50 60 MSKKTVASLS AADISGKRAL VRVDFNVPLD DQGNITDDTR IRAALPTIQD LTQKGAKVIL 70 80 90 100 110 120 ASHFGRPKGV DEKLRLTPVA KRLSELLGQE VIKTDDSIGD EVAAKVATLQ NGQVLLLENV 130 140 150 160 170 180 RFYKEEEKND PEFAKKLAAN ADFYVNDAFG TAHRAHASTE GVTKFLSPSV AGYLVEKELQ 190 200 210 220 230 240 YLQSAIENPQ RPLAAIIGGS KVSSKIGVIE TLLEKCDKLI IGGGMIFTFY KARGLNVGKS 250 260 270 280 290 300 LVEEDKLELA KSLEAKAKER GVSLLLPTDV VLADNFAPDA NSQTVSIENI PDGWMGLDIG 310 320 330 340 350 360 PDSVKVFQAA LADTKTVIWN GPMGVFEFDK FAAGTEAIAH TLAEIGKTGT TTIIGGGDSV 370 380 390 400 AAVEKVGLAD QMSHISTGGG ASLELLEGKV LPGIAALDEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.
[2]	Singh H., Rajaram H., Apte S.K. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 306-315, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB75830.1.
PIR	AD2322.
RefSeq	NP_488171.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YPR1.
SMR	Q8YPR1. Positions 1-399.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YPR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1107733.
GenomeReviews	Gene locus all4131 in contig BA000019_GR.
KEGG	ana:all4131.
NMPDR	fig|103690.1.peg.4438.
PATRIC	22778815. VBINosSp37423_4856.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HBG453500.
OMA	DCIGDEV.
PhylomeDB	Q8YPR1.
ProtClustDB	PRK00073.
Enzyme and pathway databases
BioCyc	NSP103690:ALL4131-MONOMER.
Family and domain databases
HAMAP	MF_00145. Phosphoglyc_kinase. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
KO	K00927.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_NOSS1

Accession

Primary (citable) accession number: Q8YPR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program