Q8YPF9 (ARGJ2_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine biosynthesis bifunctional protein ArgJ 2

Cleaved into the following 2 chains:

1. Arginine biosynthesis bifunctional protein ArgJ alpha chain
2. Arginine biosynthesis bifunctional protein ArgJ beta chain

Including the following 2 domains:

1. Glutamate N-acetyltransferase
   EC=2.3.1.35
   Alternative name(s):
   Ornithine acetyltransferase
   Short name=OATase
   Ornithine transacetylase
2. Amino-acid acetyltransferase
   EC=2.3.1.1
   Alternative name(s):
   N-acetylglutamate synthase
   Short name=AGSase

Gene names

Name:	argJ2
Ordered Locus Names:	alr4235

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate By similarity. HAMAP-Rule MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP-Rule MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP-Rule MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP-Rule MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP-Rule MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01106.
Miscellaneous	Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP-Rule MF_01106
Sequence similarities	Belongs to the ArgJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Autocatalytic cleavage
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Arginine biosynthesis bifunctional protein ArgJ alpha chain By similarity		PRO_0000002099
	Chain	180 – 387	208	Arginine biosynthesis bifunctional protein ArgJ beta chain By similarity		PRO_0000002100
Sites
	Active site	180	1	Nucleophile By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
	Binding site	382	1	Substrate By similarity
	Binding site	387	1	Substrate By similarity
	Site	110	1	Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole By similarity
	Site	111	1	Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole By similarity
	Site	179 – 180	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YPF9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: B4A340DC3FF3013D	FASTA	387	41,146
	10 20 30 40 50 60 MSLTASSTPQ GFSTFITNLG IRDTTEDFVF LRSDVPCVAD GVFTQSLFAG PSVTISRQNL 70 80 90 100 110 120 QDGQAQGIII ISKNANVANG AVGIADAQEI IQLVAQETGI AAENLAIAST GVIGRRYPIE 130 140 150 160 170 180 KIRAGLVGMG QKLTAADFDL AARGIMTTDT VSKIAARQVG NAKLVGIAKG VGMIEPNMAT 190 200 210 220 230 240 MLAFFFTDAA ISANTLRQIF RSTIDKTFNC LSIDTDTSTS DSAVILANGL AGEVPEAEFA 250 260 270 280 290 300 SALQEIAHDL VLKIARDGEG ATKVIEVTVD SAANYAQAKR VAKAIVNSPL VKTAVYGADP 310 320 330 340 350 360 NWGRVAMAIG KCEDERDINP DQVVIRFDEV QVYPNTFQAE NLEKLKEIMS KEKVNIHVSL 370 380 NIGTDVATVW GCDLTEGYVE INGKYST

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB75934.1.
PIR	AD2335.
RefSeq	NP_488275.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YPF9.
SMR	Q8YPF9. Positions 8-383.
ModBase	Search...
Protein-protein interaction databases
STRING	103690.alr4235.
Protein family/group databases
MEROPS	T05.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB75934; BAB75934; BAB75934.
GeneID	1107837.
KEGG	ana:alr4235.
PATRIC	22779027. VBINosSp37423_4961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1364.
HOGENOM	HOG000022797.
KO	K00620.
OMA	IEENAYY.
ProtClustDB	PRK05388.
Enzyme and pathway databases
UniPathway	UPA00068; UER00106. UPA00068; UER00111.
Family and domain databases
Gene3D	3.60.70.12. 1 hit.
HAMAP	MF_01106. ArgJ.
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. ArgJ-like_dom. [Graphical view]
PANTHER	PTHR23100. PTHR23100. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. Arg_biosynth_ArgJ. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56266. Pept_S58_DmpA/Arg_biosyn_ArgJ. 1 hit.
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ2_NOSS1

Accession

Primary (citable) accession number: Q8YPF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program