Reviewed, UniProtKB/Swiss-Prot Q8YP78 (MDH_ANASP)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	alr4322

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Malate dehydrogenase HAMAP MF_00487		PRO_0000113421
Regions
	Nucleotide binding	20 – 25	6	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	186	1	Proton acceptor By similarity
	Binding site	44	1	NAD By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	106	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YP78-1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 081EEA3377A457B7	FASTA	323	34,924
	10 20 30 40 50 60 MFSSPDSPIL HCLPRVAIIG AGRVGSTLAQ RIAEKNLADV VLLDIVEGIP QGLALDLLEA 70 80 90 100 110 120 RGIELHNRQI IGTNNYADTS GSQIVVITAG FPRKPGMSRD DLLRTNAKIV IEAAKQAIAY 130 140 150 160 170 180 SPYAIFIVVT NPLDVMTYLA WEATGLPRNR IMGMAGVLDS ARFETFIALE LGVLPADVKA 190 200 210 220 230 240 MVLGSHGDLM VPLSRYATVN GIPITQLLDA VTIERLVERT RNGGAEIVEL MQTGGAFFAP 250 260 270 280 290 300 ASATSLMVES ILLNQSRLLP VSIYLQGEYD LKDVVIGVPC RLGLNGIESV IELNLSDSER 310 320 EALHISAKSV QKNIERWRSL QNS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB76021.1. Different initiation.
PIR	AC2346.
RefSeq	NP_488362.1.
3D structure databases
HSSP	HSSP built from PDB template 1GV0 based on UniProtKB P80039.
ModBase	Search...
Genome annotation databases
GeneID	1107924.
GenomeReviews	Gene locus alr4322 in contig BA000019_GR.
KEGG	ana:alr4322.
NMPDR	fig|103690.1.peg.4629.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YP78.
Enzyme and pathway databases
BioCyc	NSP103690:ALR4322-MON.
Family and domain databases
HAMAP	MF_00487. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_NAD-dep_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_ANASP

Accession

Primary (citable) accession number: Q8YP78

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)