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Protein

LL-diaminopimelate aminotransferase 2

Gene

dapL2

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli (By similarity).By similarity

Catalytic activityi

LL-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Substrate; via amide nitrogenBy similarity
Binding sitei64 – 641Pyridoxal phosphate; shared with dimeric partnerBy similarity
Binding sitei127 – 1271SubstrateBy similarity
Binding sitei177 – 1771Pyridoxal phosphateBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Binding sitei205 – 2051Pyridoxal phosphateBy similarity
Binding sitei208 – 2081Pyridoxal phosphateBy similarity
Binding sitei236 – 2361Pyridoxal phosphateBy similarity
Binding sitei238 – 2381Pyridoxal phosphateBy similarity
Binding sitei247 – 2471Pyridoxal phosphateBy similarity
Binding sitei365 – 3651SubstrateBy similarity

GO - Molecular functioni

  1. L,L-diaminopimelate aminotransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate, diaminopimelate-aminotransferase pathway Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00034; UER00466.

Names & Taxonomyi

Protein namesi
Recommended name:
LL-diaminopimelate aminotransferase 2 (EC:2.6.1.83)
Short name:
DAP-AT 2
Short name:
DAP-aminotransferase 2
Short name:
LL-DAP-aminotransferase 2
Gene namesi
Name:dapL2
Ordered Locus Names:all4327
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390LL-diaminopimelate aminotransferase 2PRO_0000342210Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei239 – 2391N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi103690.all4327.

Structurei

3D structure databases

ProteinModelPortaliQ8YP73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0436.
HOGENOMiHOG000223051.
KOiK10206.
OMAiLCHDHAY.
OrthoDBiEOG6W721W.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01642. DapL_aminotrans_1.
InterProiIPR004839. Aminotransferase_I/II.
IPR019942. DapL_aminotrans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YP73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFAKRLEKI PPYLFAEINR KREALIAKGV DIINIGVGDP DKPTPAHILQ
60 70 80 90 100
AMREAIDDAS NHNYPPYEGT QEFREAAVEW MERRFGVMDL NPNTEVVSSI
110 120 130 140 150
GSKEAIHNTF LAFVEAGDYT LIPDPGYPVY RTSTIFAGGE AFSMPLKAEN
160 170 180 190 200
KFLPDLDLIP EEVARKAKML WINYPNNPTG ALATLEFFEE LVALCQQYSI
210 220 230 240 250
LLCHDHAYSE MAYDGYKPPS VLQIPGAKDI AIEFHSLSKS YNMTGWRIGF
260 270 280 290 300
AVGNAYAIKG LSQVKTNVDS GVFKAIQKAA IAAYATDEVE LQAVMSVYQS
310 320 330 340 350
RRDIIVKGLQ SLGWPIEPPK ATLYVWVPVP PGYTSTEFTT LLLDKCGIVV
360 370 380 390
PPGVGYGASG EGYFRVALTI SDERLHEAIQ RMQDAGIRYA
Length:390
Mass (Da):43,225
Last modified:March 1, 2002 - v1
Checksum:i6CFB39A6C8C32E8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB76026.1.
PIRiAH2346.
RefSeqiNP_488367.1. NC_003272.1.
WP_010998465.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB76026; BAB76026; BAB76026.
GeneIDi1107929.
KEGGiana:all4327.
PATRICi22779226. VBINosSp37423_5060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB76026.1.
PIRiAH2346.
RefSeqiNP_488367.1. NC_003272.1.
WP_010998465.1. NC_003272.1.

3D structure databases

ProteinModelPortaliQ8YP73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.all4327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB76026; BAB76026; BAB76026.
GeneIDi1107929.
KEGGiana:all4327.
PATRICi22779226. VBINosSp37423_5060.

Phylogenomic databases

eggNOGiCOG0436.
HOGENOMiHOG000223051.
KOiK10206.
OMAiLCHDHAY.
OrthoDBiEOG6W721W.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00466.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01642. DapL_aminotrans_1.
InterProiIPR004839. Aminotransferase_I/II.
IPR019942. DapL_aminotrans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiDAPT2_NOSS1
AccessioniPrimary (citable) accession number: Q8YP73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.