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Q8YNK2 (ALF_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase

Short name=FBP aldolase
Short name=FBPA
EC=4.1.2.13
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene names
Name:fda
Ordered Locus Names:all4563
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity. UniProtKB Q55664

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. UniProtKB Q55664

Cofactor

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity. UniProtKB Q55664

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. UniProtKB Q55664

Sequence similarities

Belongs to the class II fructose-bisphosphate aldolase family.

Mass spectrometry

Molecular mass is 38764±1 Da from positions 1 - 359. Determined by MALDI. Ref.2

Ontologies

Keywords
   Biological processGlycolysis
   LigandMetal-binding
Zinc
   Molecular functionLyase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity UniProtKB Q55664
Chain2 – 359358Fructose-bisphosphate aldolase UniProtKB Q55664
PRO_0000366199

Regions

Region233 – 2353Dihydroxyacetone phosphate binding By similarity UniProtKB Q55664
Region275 – 2784Dihydroxyacetone phosphate binding By similarity UniProtKB Q55664

Sites

Active site831Proton donor By similarity UniProtKB Q55664
Metal binding841Zinc 1; catalytic By similarity UniProtKB Q55664
Metal binding1051Zinc 2 By similarity UniProtKB Q55664
Metal binding1421Zinc 2 By similarity UniProtKB Q55664
Metal binding1981Zinc 1; catalytic By similarity UniProtKB Q55664
Metal binding2321Zinc 1; catalytic By similarity UniProtKB Q55664
Binding site501Glyceraldehyde 3-phosphate By similarity UniProtKB Q55664
Binding site1991Dihydroxyacetone phosphate; via amide nitrogen By similarity UniProtKB Q55664

Sequences

Sequence LengthMass (Da)Tools
Q8YNK2 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: E91D84D7591007B1

FASTA35938,617
        10         20         30         40         50         60 
MALVPLRLLL DHAAENGYGI PAFNVNNLEQ IQAILKAAAE TDSPVILQAS RGARNYAGEN 

        70         80         90        100        110        120 
FLRHLILAAV ETYPEIPIVM HQDHGNAPST CYSAIKNNFT SVMMDGSLEA DAKTPASFEY 

       130        140        150        160        170        180 
NVNVTREVVN VAHALGVSVE GELGCLGSLE TGAGEAEDGH GFEGTLDHSQ LLTDPDEAVN 

       190        200        210        220        230        240 
FVEATQVDAL AVAIGTSHGA YKFTRKPTGE ILAISRIEEI HRRLPNTHLV MHGSSSVPED 

       250        260        270        280        290        300 
LIALINEYGG AIPETYGVPV EEIQKGIKSG VRKVNIDTDN RLAITAAVRE ALAKNPKEFD 

       310        320        330        340        350 
PRHFLKPSIT YMQKVCAERY VQFGTAGNAS KIKQVSLETF AAKYAKGELN AISKAAAKV 

« Hide

References

« Hide 'large scale' references
[1]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.
[2]Singh H., Rajaram H., Apte S.K.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 282-289, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000019 Genomic DNA. Translation: BAB76262.1.
PIRAC2376.
RefSeqNP_488603.1. NC_003272.1.

3D structure databases

ProteinModelPortalQ8YNK2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.all4563.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB76262; BAB76262; BAB76262.
GeneID1108164.
KEGGana:all4563.
PATRIC22779738. VBINosSp37423_5315.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0191.
HOGENOMHOG000227792.
KOK01624.
OMACISAITN.
OrthoDBEOG6HXJ7B.

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR006412. Fruct_bisP_Calv.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsTIGR00167. cbbA. 1 hit.
TIGR01521. FruBisAldo_II_B. 1 hit.
PROSITEPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_NOSS1
AccessionPrimary (citable) accession number: Q8YNK2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways