Q8YNK2 (ALF_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase
Short name=FBP aldolase
Short name=FBPA
EC=4.1.2.13

Alternative name(s):
Fructose-1,6-bisphosphate aldolase

Gene names

Name:	fda
Ordered Locus Names:	all4563

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity. UniProtKB Q55664
Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. UniProtKB Q55664
Cofactor	Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity. UniProtKB Q55664
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. UniProtKB Q55664
Sequence similarities	Belongs to the class II fructose-bisphosphate aldolase family.
Mass spectrometry	Molecular mass is 38764±1 Da from positions 1 - 359. Determined by MALDI. Ref.2

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity UniProtKB Q55664
	Chain	2 – 359	358	Fructose-bisphosphate aldolase UniProtKB Q55664		PRO_0000366199
Regions
	Region	233 – 235	3	Dihydroxyacetone phosphate binding By similarity UniProtKB Q55664
	Region	275 – 278	4	Dihydroxyacetone phosphate binding By similarity UniProtKB Q55664
Sites
	Active site	83	1	Proton donor By similarity UniProtKB Q55664
	Metal binding	84	1	Zinc 1; catalytic By similarity UniProtKB Q55664
	Metal binding	105	1	Zinc 2 By similarity UniProtKB Q55664
	Metal binding	142	1	Zinc 2 By similarity UniProtKB Q55664
	Metal binding	198	1	Zinc 1; catalytic By similarity UniProtKB Q55664
	Metal binding	232	1	Zinc 1; catalytic By similarity UniProtKB Q55664
	Binding site	50	1	Glyceraldehyde 3-phosphate By similarity UniProtKB Q55664
	Binding site	199	1	Dihydroxyacetone phosphate; via amide nitrogen By similarity UniProtKB Q55664

Sequences

	Sequence		Length	Mass (Da)
	Q8YNK2 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E91D84D7591007B1	FASTA	359	38,617
	10 20 30 40 50 60 MALVPLRLLL DHAAENGYGI PAFNVNNLEQ IQAILKAAAE TDSPVILQAS RGARNYAGEN 70 80 90 100 110 120 FLRHLILAAV ETYPEIPIVM HQDHGNAPST CYSAIKNNFT SVMMDGSLEA DAKTPASFEY 130 140 150 160 170 180 NVNVTREVVN VAHALGVSVE GELGCLGSLE TGAGEAEDGH GFEGTLDHSQ LLTDPDEAVN 190 200 210 220 230 240 FVEATQVDAL AVAIGTSHGA YKFTRKPTGE ILAISRIEEI HRRLPNTHLV MHGSSSVPED 250 260 270 280 290 300 LIALINEYGG AIPETYGVPV EEIQKGIKSG VRKVNIDTDN RLAITAAVRE ALAKNPKEFD 310 320 330 340 350 PRHFLKPSIT YMQKVCAERY VQFGTAGNAS KIKQVSLETF AAKYAKGELN AISKAAAKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.
[2]	Singh H., Rajaram H., Apte S.K. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 282-289, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB76262.1.
PIR	AC2376.
RefSeq	NP_488603.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YNK2.
ModBase	Search...
Protein-protein interaction databases
STRING	103690.all4563.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB76262; BAB76262; BAB76262.
GeneID	1108164.
KEGG	ana:all4563.
PATRIC	22779738. VBINosSp37423_5315.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0191.
HOGENOM	HOG000227792.
KO	K01624.
OMA	VNAPVIM.
ProtClustDB	PRK09196.
Enzyme and pathway databases
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR006412. Fruct_bisP_Calv. IPR000771. Ketose_bisP_aldolase_II. [Graphical view]
Pfam	PF01116. F_bP_aldolase. 1 hit. [Graphical view]
PIRSF	PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMs	TIGR00167. cbbA. 1 hit. TIGR01521. FruBisAldo_II_B. 1 hit.
PROSITE	PS00602. ALDOLASE_CLASS_II_1. 1 hit. PS00806. ALDOLASE_CLASS_II_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF_NOSS1

Accession

Primary (citable) accession number: Q8YNK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program