Q8YN97 (KPRS_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	alr4670

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141104
Regions
	Region	230 – 243	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	146	1	Magnesium Potential
	Metal binding	148	1	Magnesium Potential
	Metal binding	157	1	Magnesium Potential
	Metal binding	161	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YN97 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4C7E088BE4E4C797	FASTA	330	36,148
	10 20 30 40 50 60 MLSSATFKLQ PTATGLTENH RLRLFSGSSN VQLSQEVARY LGMDLGPMIR KRFADGELYV 70 80 90 100 110 120 QIQESIRGCD VYLIQPCCQP VNDHLMELLI MVDACRRASA RQVTAVIPYY GYARADRKTA 130 140 150 160 170 180 GRESITAKLV ANLITQAGAN RVLAMDLHAA QIQGYFDIPF DHVYGSPVLL DYLTSKQLHD 190 200 210 220 230 240 IVVVSPDVGG VARARAFAKK LNDAPLAIID KRRQAHNVAE VLNVIGDVKG KTAVLVDDMI 250 260 270 280 290 300 DTGGTIAAGA KLLREEGARQ VYACATHAVF SPPAVERLSS GLFEEVIVTN TIPIPESDRF 310 320 330 PQLVVLSVAN LLGETIWRIH EDTSISSMFR

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB76369.1.
PIR	AF2389.
RefSeq	NP_488710.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YN97.
SMR	Q8YN97. Positions 22-330.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YN97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1108271.
GenomeReviews	Gene locus alr4670 in contig BA000019_GR.
KEGG	ana:alr4670.
NMPDR	fig|103690.1.peg.4977.
PATRIC	22779964. VBINosSp37423_5426.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0462.
HOGENOM	HBG519284.
OMA	CATHAVF.
PhylomeDB	Q8YN97.
ProtClustDB	PRK02812.
Enzyme and pathway databases
BioCyc	NSP103690:ALR4670-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_NOSS1

Accession

Primary (citable) accession number: Q8YN97

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program