Reviewed, UniProtKB/Swiss-Prot Q8YMZ0 (PURA_ANASP)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	alr4784

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000095141
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	139	1	By similarity
	Active site	146	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YMZ0-1 [UniParc]. Last modified April 23, 2003. Version 2. Checksum: E7A3144919DA3D4A	FASTA	447	49,206
	10 20 30 40 50 60 MANVIVIGAQ WGDEGKGKIT DLLSRSADVV VRYQGGVNAG HTIVVKGQTF KLHLIPSGIL 70 80 90 100 110 120 YPDTECMIGC GTVIDPQVLI KELDQLESLN ISTKNLLISE TAHVTMPYHR LIDKASEERR 130 140 150 160 170 180 GSHKIGTTGR GIGPTYADKS ERTGIRVLDL MDPAALRDQL EWTINNKNVI LEKLYNLPPL 190 200 210 220 230 240 DTEEVIQEYI GYAERLRPHV VDTSLKIYDA IQRRRNILFE GAQGTLLDLD HGTYPYVTSS 250 260 270 280 290 300 NPVAGGACVG TGLGPTMIDR VIGVSKAYTT RVGEGPFPTE LHGELGELLC DRGAEFGTTT 310 320 330 340 350 360 GRKRRCGWFD AVIGRYAVRI NGMDCMAITK LDVLDELEEI QVCIAYEIDG DRCDHFPTSA 370 380 390 400 410 420 RQFARCRPIY KTVPGWQVPT SECRTLEDLP QQALDYLKFL AELMEVPIAI VSLGASRDQT 430 440 IIVEDPIHGP KRALLHPDGT PASLLSA

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB76483.1. Different initiation.
PIR	AH2403.
RefSeq	NP_488824.1.
3D structure databases
HSSP	HSSP built from PDB template 1ADE based on UniProtKB P12283.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YMZ0.
Genome annotation databases
GeneID	1108385.
GenomeReviews	Gene locus alr4784 in contig BA000019_GR.
KEGG	ana:alr4784.
NMPDR	fig|103690.1.peg.5091.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YMZ0.
Enzyme and pathway databases
BioCyc	NSP103690:ALR4784-MON.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ANASP

Accession

Primary (citable) accession number: Q8YMZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	September 1, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)