Reviewed, UniProtKB/Swiss-Prot Q8YMX6 (ASSY_ANASP)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	alr4798

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148561
Regions
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Binding site	38	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	89	1	Citrulline By similarity
	Binding site	119	1	ATP; via amide nitrogen By similarity
	Binding site	121	1	Aspartate By similarity
	Binding site	125	1	Aspartate By similarity
	Binding site	125	1	Citrulline By similarity
	Binding site	126	1	Aspartate By similarity
	Binding site	129	1	Citrulline By similarity
	Binding site	177	1	Citrulline By similarity
	Binding site	186	1	Citrulline By similarity
	Binding site	262	1	Citrulline By similarity
	Binding site	274	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YMX6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9F84B384A0EC50EC	FASTA	400	43,842
	10 20 30 40 50 60 MGRAKKVVLA YSGGVDTSVC IPYLKQEWGV EEVITLAADL GQGDELEPIR EKALKSGASE 70 80 90 100 110 120 SLVADVKESF IKDYAFPAIQ ANALYENRYP LGTALARPLI AKILVEAAAK YGADAIAHGC 130 140 150 160 170 180 TGKGNDQVRF DVSCTALNPN LKILAPAREW GMSREATIAY GEKFGIPSPV KKSSPYSIDK 190 200 210 220 230 240 NLLGRSIEAG ALEDPKFEPP EEIYEMTKAI ADTPNEPEYI EIGFTQGLPT TLNGIPKDPV 250 260 270 280 290 300 ALIQELNQVV GSHGVGRIDM IENRLVGIKS REIYESPAML VLIPAHRDLE SLTLTADVSH 310 320 330 340 350 360 YKRGIEETYS QIVYNGLWYS PLKAALDAFI QKTQERVSGT VRVKLFKGNA TIVGRWSDSS 370 380 390 400 LYTPDLATYG AEDQFDHKAA EGFIYVWGLP TRIWAQQDRG

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB76497.1.
PIR	AF2405.
RefSeq	NP_488838.1.
3D structure databases
HSSP	HSSP built from PDB template 1KP2 based on UniProtKB P22767.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YMX6.
Genome annotation databases
GeneID	1108399.
GenomeReviews	Gene locus alr4798 in contig BA000019_GR.
KEGG	ana:alr4798.
NMPDR	fig|103690.1.peg.5105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YMX6.
OMA	IAPVREW.
Enzyme and pathway databases
BioCyc	NSP103690:ALR4798-MON.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_ANASP

Accession

Primary (citable) accession number: Q8YMX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)