Q8YMT4 (SYFA_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	all4845

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000126656
Sites
	Metal binding	257	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YMT4 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1DCE9E4E5DB6723A	FASTA	330	37,119
	10 20 30 40 50 60 MTSNLEAQLL ALRQEGEQAI AAADTLERLE ELRVSYLGKK GQLGALLRSM GQMSAEERPK 70 80 90 100 110 120 IGAIANTVKE ALQASLDKQR ESLESAQIQA QLDAETLDVT MPGIYKPQGR IHPLNGIIDR 130 140 150 160 170 180 ALDVFVGLGY TVAQGLEMET DYYNFEALNT PPDHPARDMQ DTFYLPDGNL LRTHTSSVQI 190 200 210 220 230 240 RYMEKEEPPI HIVAPGRVYR RDNVDATHSA VFHQIELLAI DEGLTFTDLK GTIKVFLQAM 250 260 270 280 290 300 FGDLPIRFRA SYFPFTEPSA EVDLQWNGRW LEVMGCGMVD PNVMKSVGYN PEIYTGFAAG 310 320 330 FGVERFAMVL HQIDDIRRLY ASDLRFLRQF

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB76544.1.
PIR	AE2411.
RefSeq	NP_488885.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YMT4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YMT4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1108446.
GenomeReviews	Gene locus all4845 in contig BA000019_GR.
KEGG	ana:all4845.
NMPDR	fig|103690.1.peg.5152.
PATRIC	22780345. VBINosSp37423_5615.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q8YMT4.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	NSP103690:ALL4845-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_NOSS1

Accession

Primary (citable) accession number: Q8YMT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program