Reviewed, UniProtKB/Swiss-Prot Q8YM83 (SYP_ANASP)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	alr5053

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 604	604	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000248638

Sequences

	Sequence		Length	Mass (Da)
	Q8YM83-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 796295D88F314E3C	FASTA	604	67,646
	10 20 30 40 50 60 MRLSQMLFVT LRDDPSDAEI PSHKLLLRAG YIRRIGSGIY AYLPLMWRVL QKVSQIVREE 70 80 90 100 110 120 MNATGAQECL LPQLQPSELW KESGRWDTYT KAEGIMFSLI DRREQQLGLG PTHEEVITAI 130 140 150 160 170 180 ARDMIRSYRQ LPLHLYQLQT KFRDEIRPRF GLMRGREFIM KDGYSFHVDE DSLKKTYQDM 190 200 210 220 230 240 YQAYSNMLRR AGLAFRPVEA DSGAIGGSGS TEFMVLAEAG EDEVLYTDDG KYAANVEKAV 250 260 270 280 290 300 SLPADAEPSQ FTTFEKRETP GTETIEKVTQ FLKASPTQIV KNVLYQTAYD NGVTVLVLVI 310 320 330 340 350 360 IRGDQEVNEV KLQNELTKLA ANYGAKAIIS LTVPSAENQQ TWTAKPLPLG YIAPNIADEY 370 380 390 400 410 420 IAANKQIHPK FVRFVDKTVV DLKNFITGAN EAGCHVVGAN WGEQFPLPEI VVDVRKARPG 430 440 450 460 470 480 DRAVHDSTQL LKSARGIEVG HIFQLGTKYS QALGATYTNE QGEEKPLVMG CYGVGVSRLA 490 500 510 520 530 540 QSAVEQSYDK DGIIWPVAIA PYHAIVTIPN INDAQQVEIA EKLYTELNQS GVETLLDDRN 550 560 570 580 590 600 ERAGVKFKDA DLIGIPYRIV TGRAITNGKV EIVERATRQS QEIPIDEVIT TLQQWIRAAI EQKN

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB76752.1.
PIR	AE2437.
RefSeq	NP_489093.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YM83.
Genome annotation databases
GeneID	1108657.
GenomeReviews	Gene locus alr5053 in contig BA000019_GR.
KEGG	ana:alr5053.
NMPDR	fig|103690.1.peg.5360.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	DFVLGPT.
PhylomeDB	Q8YM83.
Enzyme and pathway databases
BioCyc	NSP103690:ALR5053-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ANASP

Accession

Primary (citable) accession number: Q8YM83

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)