Reviewed, UniProtKB/Swiss-Prot Q8YM75 (MURC_ANASP)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	alr5065

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182045
Regions
	Nucleotide binding	140 – 146	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YM75-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: A6312D9BEB473544	FASTA	494	53,592
	10 20 30 40 50 60 MNNSVDFGGR PFHFIGIGGI GMSALAYVLA KRQLPVSGSD LRPNHITRKL ESIGTHIFSR 70 80 90 100 110 120 QEASNLEFFG SKVSSTEIEL NTQEMFPVGK STLPQVVCST AINSNNLEYQ AAIELGCPIL 130 140 150 160 170 180 HRSDVLAALI NDYHSVAVAG THGKTTTSSM IGYMLLEAGL DPTIIVGGEV NAWEGNARLG 190 200 210 220 230 240 QSSYLVAEAD ESDGSLVKHA PEIGIITNIE LDHPDHYDTL EEVVDIFQTF AKGCKTLIGS 250 260 270 280 290 300 VDCETVRELL RNASGDRQQP TITYSLHQDT EADYTVTNID YRADGTTALV WEKGKALGVL 310 320 330 340 350 360 KLKLLSRHNL SNALAAVAVG RLVGLEFGEI AKGIAGFEGA RRRFEFRGEV DGITFIDDYA 370 380 390 400 410 420 HHPSEIRATL AAARLQARPG QRVVAIFQPH RYSRTLTFLE EFSESFSHAD LVVLTDIYSA 430 440 450 460 470 480 GEPNLGLISG ENLAEKIAQE HPQVVYQPTL STVCEYLLKN LRPGDLALFL GAGNLNQAIP 490 EIITTLCAPA TATL

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB76764.1.
PIR	AI2438.
RefSeq	NP_489105.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQY based on UniProtKB P45066.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YM75.
Genome annotation databases
GeneID	1108669.
GenomeReviews	Gene locus alr5065 in contig BA000019_GR.
KEGG	ana:alr5065.
NMPDR	fig|103690.1.peg.5372.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YM75.
OMA	EWMVVEA.
Enzyme and pathway databases
BioCyc	NSP103690:ALR5065-MON.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ANASP

Accession

Primary (citable) accession number: Q8YM75

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)