Reviewed, UniProtKB/Swiss-Prot Q8YM74 (MURB_ANASP)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	alr5066

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179170
Regions
	Domain	54 – 221	168	FAD-binding PCMH-type
Sites
	Active site	200	1	By similarity
	Active site	251	1	Proton donor By similarity
	Active site	321	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YM74-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4D523CECBDCE151A	FASTA	331	35,778
	10 20 30 40 50 60 MKISQAVGNA CTVPASSVET HNNNPSKESK IIYLPGTNCE IKSQALLSAF TSYRVGGAAE 70 80 90 100 110 120 LYVAPRNIEA LQASLRYAQE HNLRVTTLGA GSNLLVSDRG ISGLVIATRH LRYTHFDHQT 130 140 150 160 170 180 GQVTIAAGES IPSLAWEIAK LGWQGFEWAV GIPGTVGGAV VMNAGAHNSC IADILVSAQV 190 200 210 220 230 240 LSPDGTVETL TPEELGYAYR TSLLQGSNRV VTQATFQLQP GFDPAYVTAT TKQHKQMRLT 250 260 270 280 290 300 TQPYNFPSCG SVFRNPKPYS AGWLIEQSGL KGYQIGGAQV AHLHANFIVN RGGAKANDIF 310 320 330 CLIRHIQQEV QERWSILLEP EVKMLGEFQA A

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB76765.1.
PIR	AB2439.
RefSeq	NP_489106.1.
3D structure databases
HSSP	HSSP built from PDB template 1HSK based on UniProtKB Q93G02.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YM74.
Genome annotation databases
GeneID	1108670.
GenomeReviews	Gene locus alr5066 in contig BA000019_GR.
KEGG	ana:alr5066.
NMPDR	fig|103690.1.peg.5373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YM74.
OMA	GPADILI.
Enzyme and pathway databases
BioCyc	NSP103690:ALR5066-MON.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_ANASP

Accession

Primary (citable) accession number: Q8YM74

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)