Reviewed, UniProtKB/Swiss-Prot Q8YM41 (PYRE_ANASP)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	alr5099

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110664
Regions
	Region	141 – 149	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	114	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	115	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	118	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	120	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	145	1	Orotate By similarity
	Binding site	173	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YM41-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 958628E4258E250F	FASTA	206	22,199
	10 20 30 40 50 60 MTYSTESLAQ SDIWPATADV STLRRKLLDL LCQLAYKEGD FVLSSGQPSS YYINGKQVTL 70 80 90 100 110 120 HPQGALAIGR ILLSLLPSDT QAVAGLTLGA DPIVTAVSVV SAYENRPIPA LIIRKEAKGH 130 140 150 160 170 180 GTKAYIEGPN LPEGAKVVVL EDVVTTGQSA MKAVDRLRAA GYVVDEVISL VDRQQGGAEF 190 200 YQSVGLKFEA VFTIMDLQQR YQELGN

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB76798.1.
PIR	AC2443.
RefSeq	NP_489139.1.
3D structure databases
SMR	Q8YM41. Positions 22-202.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YM41.
Genome annotation databases
GeneID	1108703.
GenomeReviews	Gene locus alr5099 in contig BA000019_GR.
KEGG	ana:alr5099.
NMPDR	fig|103690.1.peg.5406.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	NCKPVSL.
PhylomeDB	Q8YM41.
Enzyme and pathway databases
BioCyc	NSP103690:ALR5099-MONOMER.
Family and domain databases
HAMAP	MF_01208_B. PyrE_B. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_ANASP

Accession

Primary (citable) accession number: Q8YM41

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)