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Q8YLG0

- CPDA_NOSS1

UniProt

Q8YLG0 - CPDA_NOSS1

Protein

3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA

Gene

cpdA

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes. Can also hydrolyze cGMP.1 PublicationUniRule annotation

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.1 PublicationUniRule annotation

    Cofactori

    Binds 2 metal cations per subunit.1 Publication

    Enzyme regulationi

    Activated by iron and manganese.1 Publication

    Kineticsi

    1. KM=45 µM for cAMP1 Publication

    Vmax=4.9 µmol/min/mg enzyme with cAMP as substrate1 Publication

    Vmax=4.4 µmol/min/mg enzyme with cGMP as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi14 – 141Metal cation 1UniRule annotation
    Metal bindingi16 – 161Metal cation 1UniRule annotation
    Binding sitei16 – 161cAMPUniRule annotation
    Metal bindingi56 – 561Metal cation 1UniRule annotation
    Metal bindingi56 – 561Metal cation 2UniRule annotation
    Binding sitei56 – 561cAMPUniRule annotation
    Metal bindingi86 – 861Metal cation 2UniRule annotation
    Metal bindingi155 – 1551Metal cation 2UniRule annotation
    Metal bindingi194 – 1941Metal cation 2UniRule annotation
    Metal bindingi196 – 1961Metal cation 1UniRule annotation
    Binding sitei196 – 1961cAMPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi86 – 872cAMPUniRule annotation

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-HAMAP
    3. nucleotide binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3',5'-cyclic adenosine monophosphate phosphodiesterase CpdAUniRule annotation (EC:3.1.4.17UniRule annotation)
    Short name:
    3',5'-cyclic AMP phosphodiesteraseUniRule annotation
    Short name:
    cAMP phosphodiesteraseUniRule annotation
    Gene namesi
    Name:cpdAUniRule annotation
    Ordered Locus Names:alr5338
    OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
    Taxonomic identifieri103690 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000002483: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2662663',5'-cyclic adenosine monophosphate phosphodiesterase CpdAPRO_0000413371Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi103690.alr5338.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YLG0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cAMP phosphodiesterase class-III family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000238351.
    KOiK03651.
    OMAiTERTLLW.
    OrthoDBiEOG6QG8GQ.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    HAMAPiMF_00905. cAMP_phophodiest_CpdA.
    InterProiIPR013622. Calcineurin-like_phos_C.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR026575. cAMP_Pdiest_CpdA.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    ProDomiPD587589. Calcineurin-like_phos_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8YLG0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNEKLPISIA QITDIHLLAS ESQRLQGIST TESFLAVMKR LEELRPELDL    50
    LLMTGDLSDD GTPESYENLQ HYLNSLQIAT YWLPGNHDCA IAMDKILNLG 100
    MVSRRKSFQR GNWNFILLNS SVTDCVYGYL SATTLDWLDS ELKMLPNNPT 150
    LIALHHPPLS VNSAWIDRSC LQNSQELFAV IDRYPQVKLV LFGHIHQEFR 200
    RQRHNVHYLG SPSTCYQFQS QSTTFAINQE LPGFRLLKLY ADGTWTTKIE 250
    RVPYSLPIEP TVTVSY 266
    Length:266
    Mass (Da):30,432
    Last modified:March 1, 2002 - v1
    Checksum:i46B3475509226736
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000019 Genomic DNA. Translation: BAB77037.1.
    PIRiAB2473.
    RefSeqiNP_489378.1. NC_003272.1.
    WP_010999462.1. NC_003272.1.

    Genome annotation databases

    EnsemblBacteriaiBAB77037; BAB77037; BAB77037.
    GeneIDi1108942.
    KEGGiana:alr5338.
    PATRICi22781413. VBINosSp37423_6142.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000019 Genomic DNA. Translation: BAB77037.1 .
    PIRi AB2473.
    RefSeqi NP_489378.1. NC_003272.1.
    WP_010999462.1. NC_003272.1.

    3D structure databases

    ProteinModelPortali Q8YLG0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 103690.alr5338.

    Protocols and materials databases

    DNASUi 1108942.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB77037 ; BAB77037 ; BAB77037 .
    GeneIDi 1108942.
    KEGGi ana:alr5338.
    PATRICi 22781413. VBINosSp37423_6142.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000238351.
    KOi K03651.
    OMAi TERTLLW.
    OrthoDBi EOG6QG8GQ.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    HAMAPi MF_00905. cAMP_phophodiest_CpdA.
    InterProi IPR013622. Calcineurin-like_phos_C.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR026575. cAMP_Pdiest_CpdA.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    ProDomi PD587589. Calcineurin-like_phos_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7120 / UTEX 2576.
    2. "Biochemical properties of a cAMP phosphodiesterase in the cyanobacterium Anabaena sp. strain PCC 7120."
      Fujisawa T., Ohmori M.
      Microbes Environ. 20:92-96(2005)
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: PCC 7120 / UTEX 2576.

    Entry informationi

    Entry nameiCPDA_NOSS1
    AccessioniPrimary (citable) accession number: Q8YLG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3