Q8YKG3 (K6PF1_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase 1
EC=2.7.1.11

Alternative name(s):
Phosphofructokinase 1
Phosphohexokinase 1

Gene names

Name:	pfkA1
Ordered Locus Names:	all7335

Encoded on

Plasmid pCC7120alpha

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Plasmid
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	6-phosphofructokinase 1 HAMAP MF_00339		PRO_0000111929
Regions
	Nucleotide binding	24 – 28	5	ATP By similarity
	Nucleotide binding	168 – 172	5	ATP By similarity
	Nucleotide binding	185 – 201	17	ATP By similarity
Sites
	Active site	141	1	Proton acceptor By similarity
	Metal binding	199	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	176	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity
	Binding site	286	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YKG3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 2D2F4851F385EE68	FASTA	359	38,288
	10 20 30 40 50 60 MSKSRRIGIL TSGGDCSGLN AVIRAVVHCA SGKGWEVFGI RQATLGLMAR PPQVSKLEID 70 80 90 100 110 120 QVDPLLTSGG TMLGTTNKGD PFAFPMPDGS FCDRSSEIIA GYHQLDLDAL IGIGGDGSLA 130 140 150 160 170 180 ILRRLAQQGA INLVGIPKTI DNDIGITEHA IGFDTAVNIA TEALDRLHFT AASHSRVMIL 190 200 210 220 230 240 EVMGRDAGHI ALAAGIAGGA DVILIPEILY SMDDICYHIK HRQEEGKNYC LIIVSEAVRT 250 260 270 280 290 300 QDGEILTLTN RLGQSRYGGI GEYLADQISD RIGAETRVTV LGHIQRGGIA SPLDRLVASA 310 320 330 340 350 FGVAAVNLIE AAKYDYMVAW QNRQVITVPI EEAIAQYKAV NPEDALVKTA RGLGIYLGE

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000020 Genomic DNA. Translation: BAB77093.1.
PIR	AG2519.
RefSeq	YP_227636.1. NC_003276.1.
3D structure databases
ProteinModelPortal	Q8YKG3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YKG3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1109376.
GenomeReviews	Gene locus all7335 in contig BA000020_GR.
KEGG	ana:all7335.
NMPDR	fig|103690.1.peg.6078.
PATRIC	22782436. VBINosSp37423_6630.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HBG405044.
OMA	NGTQKTA.
PhylomeDB	Q8YKG3.
ProtClustDB	PRK14071.
Enzyme and pathway databases
BioCyc	NSP103690:ALL7335-MONOMER.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
KO	K00850.
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_NOSS1

Accession

Primary (citable) accession number: Q8YKG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program