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Protein

ATP-dependent 6-phosphofructokinase 1

Gene

pfkA1

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
Metal bindingi116 – 1161Magnesium; catalyticUniRule annotation
Sitei117 – 1171Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei141 – 1411Proton acceptorUniRule annotation
Binding sitei176 – 1761Substrate; shared with dimeric partnerUniRule annotation
Binding sitei236 – 2361SubstrateUniRule annotation
Binding sitei277 – 2771Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 792ATPUniRule annotation
Nucleotide bindingi115 – 1184ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 1UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Alternative name(s):
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:pfkA1UniRule annotation
Ordered Locus Names:all7335
Encoded oniPlasmid pCC7120alpha0 Publication
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Plasmid pCC7120alpha

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359ATP-dependent 6-phosphofructokinase 1PRO_0000111929Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi103690.all7335.

Structurei

3D structure databases

ProteinModelPortaliQ8YKG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1413Substrate bindingUniRule annotation
Regioni183 – 1853Substrate bindingUniRule annotation
Regioni283 – 2864Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiKPAEGSM.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YKG3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKSRRIGIL TSGGDCSGLN AVIRAVVHCA SGKGWEVFGI RQATLGLMAR
60 70 80 90 100
PPQVSKLEID QVDPLLTSGG TMLGTTNKGD PFAFPMPDGS FCDRSSEIIA
110 120 130 140 150
GYHQLDLDAL IGIGGDGSLA ILRRLAQQGA INLVGIPKTI DNDIGITEHA
160 170 180 190 200
IGFDTAVNIA TEALDRLHFT AASHSRVMIL EVMGRDAGHI ALAAGIAGGA
210 220 230 240 250
DVILIPEILY SMDDICYHIK HRQEEGKNYC LIIVSEAVRT QDGEILTLTN
260 270 280 290 300
RLGQSRYGGI GEYLADQISD RIGAETRVTV LGHIQRGGIA SPLDRLVASA
310 320 330 340 350
FGVAAVNLIE AAKYDYMVAW QNRQVITVPI EEAIAQYKAV NPEDALVKTA

RGLGIYLGE
Length:359
Mass (Da):38,288
Last modified:March 1, 2002 - v1
Checksum:i2D2F4851F385EE68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000020 Genomic DNA. Translation: BAB77093.1.
PIRiAG2519.
RefSeqiWP_010999884.1. NC_003276.1.
YP_227636.1. NC_003276.1.

Genome annotation databases

EnsemblBacteriaiBAB77093; BAB77093; BAB77093.
GeneIDi1109376.
KEGGiana:all7335.
PATRICi22782436. VBINosSp37423_6630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000020 Genomic DNA. Translation: BAB77093.1.
PIRiAG2519.
RefSeqiWP_010999884.1. NC_003276.1.
YP_227636.1. NC_003276.1.

3D structure databases

ProteinModelPortaliQ8YKG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.all7335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB77093; BAB77093; BAB77093.
GeneIDi1109376.
KEGGiana:all7335.
PATRICi22782436. VBINosSp37423_6630.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiKPAEGSM.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiPFKA1_NOSS1
AccessioniPrimary (citable) accession number: Q8YKG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.