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Q8YKG3 (PFKA1_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 1

Short name=ATP-PFK 1
Short name=Phosphofructokinase 1
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 1
Gene names
Name:pfkA1
Ordered Locus Names:all7335
Encoded onPlasmid pCC7120alpha
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359ATP-dependent 6-phosphofructokinase 1 HAMAP-Rule MF_01976
PRO_0000111929

Regions

Nucleotide binding78 – 792ATP By similarity
Nucleotide binding115 – 1184ATP By similarity
Region139 – 1413Substrate binding By similarity
Region183 – 1853Substrate binding By similarity
Region283 – 2864Substrate binding By similarity

Sites

Active site1411Proton acceptor By similarity
Metal binding1161Magnesium; catalytic By similarity
Binding site141ATP; via amide nitrogen By similarity
Binding site1761Substrate; shared with dimeric partner By similarity
Binding site2361Substrate By similarity
Binding site2771Substrate; shared with dimeric partner By similarity
Site1171Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YKG3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 2D2F4851F385EE68

FASTA35938,288
        10         20         30         40         50         60 
MSKSRRIGIL TSGGDCSGLN AVIRAVVHCA SGKGWEVFGI RQATLGLMAR PPQVSKLEID 

        70         80         90        100        110        120 
QVDPLLTSGG TMLGTTNKGD PFAFPMPDGS FCDRSSEIIA GYHQLDLDAL IGIGGDGSLA 

       130        140        150        160        170        180 
ILRRLAQQGA INLVGIPKTI DNDIGITEHA IGFDTAVNIA TEALDRLHFT AASHSRVMIL 

       190        200        210        220        230        240 
EVMGRDAGHI ALAAGIAGGA DVILIPEILY SMDDICYHIK HRQEEGKNYC LIIVSEAVRT 

       250        260        270        280        290        300 
QDGEILTLTN RLGQSRYGGI GEYLADQISD RIGAETRVTV LGHIQRGGIA SPLDRLVASA 

       310        320        330        340        350 
FGVAAVNLIE AAKYDYMVAW QNRQVITVPI EEAIAQYKAV NPEDALVKTA RGLGIYLGE 

« Hide

References

[1]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000020 Genomic DNA. Translation: BAB77093.1.
PIRAG2519.
RefSeqYP_227636.1. NC_003276.1.

3D structure databases

ProteinModelPortalQ8YKG3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.all7335.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB77093; BAB77093; BAB77093.
GeneID1109376.
KEGGana:all7335.
PATRIC22782436. VBINosSp37423_6630.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248869.
KOK00850.
OMAVENRYVE.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA1_NOSS1
AccessionPrimary (citable) accession number: Q8YKG3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways