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Reviewed, UniProtKB/Swiss-Prot Q8YJS6 (MNME_BRUME)

Last modified November 3, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    tRNA modification GTPase mnmE
    EC=3.6.-.-
Gene names
Name: mnmE
Synonyms: trmE
Ordered Locus Names: BMEI0006
OrganismBrucella melitensis [Complete proteome] [HAMAP]
Taxonomic identifier29459 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity.

Cofactor

Binds 1 potassium ion per subunit By similarity.

Subunit structure

Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: HAMAP

GTPase activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

potassium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442tRNA modification GTPase mnmE HAMAP MF_00379
PRO_0000345730

Regions

Nucleotide binding231 – 2366GTP By similarity
Nucleotide binding250 – 2567GTP By similarity
Nucleotide binding275 – 2784GTP By similarity

Sites

Metal binding2351Magnesium By similarity
Metal binding2561Magnesium By similarity
Binding site271Formyltetrahydrofolate By similarity
Binding site841Formyltetrahydrofolate By similarity
Binding site1241Formyltetrahydrofolate By similarity
Binding site4421Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YJS6-1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 13607DCF455E7E80

FASTA44248,085
        10         20         30         40         50         60 
MSEIGSYHDT IFALSSGRLP SGVAVIRISG PKTRFVYETI CQAIPEPRHA ALLTFRSRNG 

        70         80         90        100        110        120 
DAIDRGLTLF FPAPHSFTGE DCAEFHLHGG KAVVEKMLAV LGELPGCRIA EAGEFTRRAF 

       130        140        150        160        170        180 
ANGKMDLTIA EGLADLIAAE TEGQRRLAMQ VASGNQRKLY SEWRQRLINA RAFIEAELDF 

       190        200        210        220        230        240 
ADESDVPGSV SMQVWQQLSA LKHEIEHHIA SGKRAAMLRD GLHVVIVGAP NAGKSSLLNF 

       250        260        270        280        290        300 
LAGRDVAIIS EEAGTTRDLL EVKLDLGGIP VYVTDTAGLR ETDSVVEKIG IERARARMAE 

       310        320        330        340        350        360 
ADLVLSLEDM SGPVSVTVEK IEAETWLIGT KADLGGSASG LWKYHISTMT GSGLEQLLDA 

       370        380        390        400        410        420 
LQAFAEAKIG QIEDAVPTRQ RHINLLRATI EEIEKAIEGD DLPLELRAEN MRLASQFLGR 

       430        440 
ITGDVDVEEI LDVIFSQFCI GK 

« Hide

Cross-references

Sequence databases

AE008917 Genomic DNA. Translation: AAL51188.1.
PIRAI3252.
RefSeqNP_538924.1.

3D structure databases

HSSPHSSP built from PDB template 1MKY based on UniProtKB Q9X1F8.
ModBaseSearch...

Genome annotation databases

GeneID1195717.
GenomeReviewsGene locus BMEI0006 in contig AE008917_GR.
KEGGbme:BMEI0006.
NMPDRfig|224914.1.peg.6.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8YJS6.
OMAKGPNSFT

Enzyme and pathway databases

BioCycBMEL224914:BMEI0006-MON.

Family and domain databases

HAMAPMF_00379.
[Tree]
InterProIPR018948. GTP_bd_TrmE_N.
IPR002917. MMR_HSR1_GTP_bd.
IPR005225. Small_GTP_bd.
IPR004520. ThdF.
[Graphical view]
Gene3DG3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
PfamPF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_BRUME
AccessionPrimary (citable) accession number: Q8YJS6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2002
Last modified: November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents