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Q8YJS6 (MNME_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA modification GTPase MnmE
EC=3.6.-.-
Gene names
Name:mnmE
Synonyms:trmE
Ordered Locus Names:BMEI0006
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00379

Cofactor

Binds 1 potassium ion per subunit By similarity. HAMAP MF_00379

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00379

Subcellular location

Cytoplasm By similarity HAMAP MF_00379.

Sequence similarities

Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily.

Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442tRNA modification GTPase MnmE HAMAP MF_00379
PRO_0000345730

Regions

Domain233 – 32290G
Nucleotide binding231 – 2366GTP By similarity
Nucleotide binding250 – 2567GTP By similarity
Nucleotide binding275 – 2784GTP By similarity

Sites

Metal binding2351Magnesium By similarity
Metal binding2561Magnesium By similarity
Binding site271Formyltetrahydrofolate By similarity
Binding site841Formyltetrahydrofolate By similarity
Binding site1241Formyltetrahydrofolate By similarity
Binding site4421Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YJS6 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 13607DCF455E7E80

FASTA44248,085
        10         20         30         40         50         60 
MSEIGSYHDT IFALSSGRLP SGVAVIRISG PKTRFVYETI CQAIPEPRHA ALLTFRSRNG 

        70         80         90        100        110        120 
DAIDRGLTLF FPAPHSFTGE DCAEFHLHGG KAVVEKMLAV LGELPGCRIA EAGEFTRRAF 

       130        140        150        160        170        180 
ANGKMDLTIA EGLADLIAAE TEGQRRLAMQ VASGNQRKLY SEWRQRLINA RAFIEAELDF 

       190        200        210        220        230        240 
ADESDVPGSV SMQVWQQLSA LKHEIEHHIA SGKRAAMLRD GLHVVIVGAP NAGKSSLLNF 

       250        260        270        280        290        300 
LAGRDVAIIS EEAGTTRDLL EVKLDLGGIP VYVTDTAGLR ETDSVVEKIG IERARARMAE 

       310        320        330        340        350        360 
ADLVLSLEDM SGPVSVTVEK IEAETWLIGT KADLGGSASG LWKYHISTMT GSGLEQLLDA 

       370        380        390        400        410        420 
LQAFAEAKIG QIEDAVPTRQ RHINLLRATI EEIEKAIEGD DLPLELRAEN MRLASQFLGR 

       430        440 
ITGDVDVEEI LDVIFSQFCI GK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE008917 Genomic DNA. Translation: AAL51188.1.
PIRAI3252.
RefSeqNP_538924.1. NC_003317.1.

3D structure databases

HSSPHSSP built from PDB template 1MKY based on UniProtKB Q9X1F8.
ProteinModelPortalQ8YJS6.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1195717.
GenomeReviewsGene locus BMEI0006 in contig AE008917_GR.
KEGGbme:BMEI0006.
NMPDRfig|224914.1.peg.6.
PATRIC17795699. VBIBruMel146950_1121.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG746795.
OMAREVAIVS.
PhylomeDBQ8YJS6.
ProtClustDBPRK05291.

Enzyme and pathway databases

BioCycBMEL224914:BMEI0006-MONOMER.

Family and domain databases

HAMAPMF_00379. GTPase_MnmE.
[Tree]
InterProIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_TrmE.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
Gene3DG3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
KOK03650.
PfamPF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00450. MnmE_trmE_thdF. 1 hit.
TIGR00231. Small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_BRUME
AccessionPrimary (citable) accession number: Q8YJS6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2002
Last modified: January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

SIMILARITY comments

Index of protein domains and families

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