Reviewed, UniProtKB/Swiss-Prot Q8YJS6 (MNME_BRUME)

Names and origin

Protein names

Recommended name:
    tRNA modification GTPase mnmE
    EC=3.6.-.-

Gene names

Name:	mnmE
Synonyms:	trmE
Ordered Locus Names:	BMEI0006

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity.
Cofactor	Binds 1 potassium ion per subunit By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	tRNA modification GTPase mnmE HAMAP MF_00379		PRO_0000345730
Regions
	Nucleotide binding	231 – 236	6	GTP By similarity
	Nucleotide binding	250 – 256	7	GTP By similarity
	Nucleotide binding	275 – 278	4	GTP By similarity
Sites
	Metal binding	235	1	Magnesium By similarity
	Metal binding	256	1	Magnesium By similarity
	Binding site	27	1	Formyltetrahydrofolate By similarity
	Binding site	84	1	Formyltetrahydrofolate By similarity
	Binding site	124	1	Formyltetrahydrofolate By similarity
	Binding site	442	1	Formyltetrahydrofolate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJS6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 13607DCF455E7E80	FASTA	442	48,085
	10 20 30 40 50 60 MSEIGSYHDT IFALSSGRLP SGVAVIRISG PKTRFVYETI CQAIPEPRHA ALLTFRSRNG 70 80 90 100 110 120 DAIDRGLTLF FPAPHSFTGE DCAEFHLHGG KAVVEKMLAV LGELPGCRIA EAGEFTRRAF 130 140 150 160 170 180 ANGKMDLTIA EGLADLIAAE TEGQRRLAMQ VASGNQRKLY SEWRQRLINA RAFIEAELDF 190 200 210 220 230 240 ADESDVPGSV SMQVWQQLSA LKHEIEHHIA SGKRAAMLRD GLHVVIVGAP NAGKSSLLNF 250 260 270 280 290 300 LAGRDVAIIS EEAGTTRDLL EVKLDLGGIP VYVTDTAGLR ETDSVVEKIG IERARARMAE 310 320 330 340 350 360 ADLVLSLEDM SGPVSVTVEK IEAETWLIGT KADLGGSASG LWKYHISTMT GSGLEQLLDA 370 380 390 400 410 420 LQAFAEAKIG QIEDAVPTRQ RHINLLRATI EEIEKAIEGD DLPLELRAEN MRLASQFLGR 430 440 ITGDVDVEEI LDVIFSQFCI GK

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51188.1.
PIR	AI3252.
RefSeq	NP_538924.1.
3D structure databases
HSSP	HSSP built from PDB template 1MKY based on UniProtKB Q9X1F8.
ModBase	Search...
Genome annotation databases
GeneID	1195717.
GenomeReviews	Gene locus BMEI0006 in contig AE008917_GR.
KEGG	bme:BMEI0006.
NMPDR	fig|224914.1.peg.6.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJS6.
OMA	KGPNSFT
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0006-MON.
Family and domain databases
HAMAP	MF_00379. [Tree]
InterPro	IPR018948. GTP_bd_TrmE_N. IPR002917. MMR_HSR1_GTP_bd. IPR005225. Small_GTP_bd. IPR004520. ThdF. [Graphical view]
Gene3D	G3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
Pfam	PF01926. MMR_HSR1. 1 hit. PF10396. TrmE_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00450. mnmE_trmE_thdF. 1 hit. TIGR00231. small_GTP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNME_BRUME

Accession

Primary (citable) accession number: Q8YJS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)