Q8YJS6 (MNME_BRUME) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 68.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: tRNA modification GTPase MnmE EC=3.6.-.- | ||||||
| Gene names |
| ||||||
| Organism | Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 224914 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 442 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00379 |
| Cofactor | Binds 1 potassium ion per subunit By similarity. HAMAP MF_00379 |
| Subunit structure | Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00379 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00379. |
| Sequence similarities | Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily. Contains 1 G (guanine nucleotide-binding) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | tRNA processing |
| Cellular component | Cytoplasm |
| Ligand | GTP-binding Magnesium Metal-binding Nucleotide-binding Potassium |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | tRNA modification Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activityInferred from electronic annotation. Source: InterPro metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 442 | 442 | tRNA modification GTPase MnmE HAMAP MF_00379 | PRO_0000345730 | |||||
Regions | |||||||||
| Domain | 233 – 322 | 90 | G | ||||||
| Nucleotide binding | 231 – 236 | 6 | GTP By similarity | ||||||
| Nucleotide binding | 250 – 256 | 7 | GTP By similarity | ||||||
| Nucleotide binding | 275 – 278 | 4 | GTP By similarity | ||||||
Sites | |||||||||
| Metal binding | 235 | 1 | Magnesium By similarity | ||||||
| Metal binding | 256 | 1 | Magnesium By similarity | ||||||
| Binding site | 27 | 1 | Formyltetrahydrofolate By similarity | ||||||
| Binding site | 84 | 1 | Formyltetrahydrofolate By similarity | ||||||
| Binding site | 124 | 1 | Formyltetrahydrofolate By similarity | ||||||
| Binding site | 442 | 1 | Formyltetrahydrofolate By similarity | ||||||
Sequences
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References
| [1] | "The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. Overbeek R.Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE008917 Genomic DNA. Translation: AAL51188.1. |
| PIR | AI3252. |
| RefSeq | NP_538924.1. NC_003317.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1MKY based on UniProtKB Q9X1F8. |
| ProteinModelPortal | Q8YJS6. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1195717. |
| GenomeReviews | Gene locus BMEI0006 in contig AE008917_GR. |
| KEGG | bme:BMEI0006. |
| NMPDR | fig|224914.1.peg.6. |
| PATRIC | 17795699. VBIBruMel146950_1121. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG746795. |
| OMA | REVAIVS. |
| PhylomeDB | Q8YJS6. |
| ProtClustDB | PRK05291. |
Enzyme and pathway databases | |
| BioCyc | BMEL224914:BMEI0006-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00379. GTPase_MnmE. [Tree] |
| InterPro | IPR018948. GTP-bd_TrmE_N. IPR006073. GTP_binding_domain. IPR004520. GTPase_TrmE. IPR005225. Small_GTP-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit. |
| KO | K03650. |
| Pfam | PF01926. MMR_HSR1. 1 hit. PF10396. TrmE_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00450. MnmE_trmE_thdF. 1 hit. TIGR00231. Small_GTP. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | MNME_BRUME | ||||||||
| Accession | Primary (citable) accession number: Q8YJS6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Brucella melitensis Brucella melitensis (strain 16M): entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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