Q8YJS4 (RSMG_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.170

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase

Gene names

Name:	rsmG
Ordered Locus Names:	BMEI0008

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA By similarity. HAMAP MF_00074
Catalytic activity	S-adenosyl-L-methionine + guanosine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanosine(527) in 16S rRNA. HAMAP MF_00074
Subcellular location	Cytoplasm Potential HAMAP MF_00074.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Ribosomal RNA small subunit methyltransferase G HAMAP MF_00074		PRO_0000184229

Sequences

	Sequence		Length	Mass (Da)
	Q8YJS4 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D9B5B1C9467AB2E2	FASTA	213	23,473
	10 20 30 40 50 60 MSADIRFDSL KTIVPAVSRE TADRLIAFED LFRKWSKAIN LASPSTLADL WNRHILDSAQ 70 80 90 100 110 120 LFPLAKEATR WLDIGSGGGF PGIVTACFLA ERSGGCIDLV ESAGKKAAFL RTAAGHLHVP 130 140 150 160 170 180 ARVHSARIES MWEKIETPQV VTARALASLG DLFTLAEPWL SDGAKALFQK GRDYQREIDE 190 200 210 SRVGWSFDLV KHPSAIDQAS VILEISNLRR KTD

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51190.1.
PIR	AC3253.
RefSeq	NP_538926.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJS4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195719.
GenomeReviews	Gene locus BMEI0008 in contig AE008917_GR.
KEGG	bme:BMEI0008.
PATRIC	17795695. VBIBruMel146950_1119.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686577.
OMA	GGQGWIV.
ProtClustDB	PRK00107.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0008-MONOMER.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G. [Tree]
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
KO	K03501.
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. GidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_BRUME

Accession

Primary (citable) accession number: Q8YJS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program