Q8YJI8 (QUEC_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
7-cyano-7-deazaguanine synthase
EC=6.3.-.-

Alternative name(s):
7-cyano-7-carbaguanine synthase
PreQ(0) synthase
Queuosine biosynthesis protein QueC

Gene names

Name:	queC
Ordered Locus Names:	BMEI0095

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ0) By similarity. HAMAP MF_01633
Catalytic activity	7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O. HAMAP MF_01633
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01633
Pathway	Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. HAMAP MF_01633
Sequence similarities	Belongs to the QueC family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	7-cyano-7-deazaguanine synthase HAMAP MF_01633		PRO_0000246815
Regions
	Nucleotide binding	7 – 17	11	ATP By similarity
Sites
	Metal binding	185	1	Zinc By similarity
	Metal binding	193	1	Zinc By similarity
	Metal binding	196	1	Zinc By similarity
	Metal binding	199	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJI8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0309DDFBB3468597	FASTA	232	25,256
	10 20 30 40 50 60 MKTLVICSGG LDSVSLAHKM AAEHELTGLL SFDYGQRHKK ELDFAQACAK RLGVPHQIID 70 80 90 100 110 120 IRTIGASLTG SALTDDVDVP DGHYAEETMK VTVVPNRNAI MLAIAFGVAA AQKADAVALA 130 140 150 160 170 180 VHGGDHFIYP DCRPGFIEAF QTMQKHALDG YADVKLLAPY VHATKADIVA DGAKYRTPFE 190 200 210 220 230 ATWSCYKGAD RHCGRCGTCV ERREAFHLAG IDDPTSYEDA DFWRATTQKR NA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51277.1.
PIR	AB3264.
RefSeq	NP_539013.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJI8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195807.
GenomeReviews	Gene locus BMEI0095 in contig AE008917_GR.
KEGG	bme:BMEI0095.
PATRIC	17795500. VBIBruMel146950_1024.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553284.
OMA	DCRPDYI.
PhylomeDB	Q8YJI8.
ProtClustDB	CLSK864390.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0095-MONOMER.
Family and domain databases
HAMAP	MF_01633. QueC. [Tree]
InterPro	IPR018317. QueC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K06920.
Pfam	PF06508. ExsB. 1 hit. [Graphical view]
PIRSF	PIRSF006293. ExsB. 1 hit.
TIGRFAMs	TIGR00364. TIGR00364. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEC_BRUME

Accession

Primary (citable) accession number: Q8YJI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program