Q8YJF4 (GLO2_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	BMEI0129

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309633
Sites
	Metal binding	58	1	Zinc 1 By similarity
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	62	1	Zinc 2 By similarity
	Metal binding	63	1	Zinc 2 By similarity
	Metal binding	116	1	Zinc 1 By similarity
	Metal binding	135	1	Zinc 1 By similarity
	Metal binding	135	1	Zinc 2 By similarity
	Metal binding	173	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJF4 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: EE8B77529780D96B	FASTA	257	28,716
	10 20 30 40 50 60 MEQRLEIEQF ICRSDNYGVL IHDPESALTA TIDAPDAYAI EAALERRGWT LDFIFTTHHH 70 80 90 100 110 120 LDHVEGNEPL KEKFGVSIIG PEAEKAKIPG IDRTVKGGDE FTFGLFKVKV ISTPGHTAGG 130 140 150 160 170 180 ISYYLPDAKV VFTGDTLFAL GCGRLFEGTP ATMFHSLEKL VALPGDTALY CGHEYTQNNA 190 200 210 220 230 240 RFALTIDPDN SALKERAKEI ARLRAHERMT LPSTIALEMA TNPFLRWHDR TIRARLGLQD 250 APDEAVFAEI RKRKDMF

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51311.1.
PIR	AD3268.
RefSeq	NP_539047.1. NC_003317.1.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
ProteinModelPortal	Q8YJF4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195841.
GenomeReviews	Gene locus BMEI0129 in contig AE008917_GR.
KEGG	bme:BMEI0129.
PATRIC	17850207. VBIBruMel92729_0135.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	Q8YJF4.
ProtClustDB	CLSK898025.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0129-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_BRUME

Accession

Primary (citable) accession number: Q8YJF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program