Reviewed, UniProtKB/Swiss-Prot Q8YJF0 (DAPF_BRUME)

Names and origin

Protein names

Recommended name:
    Diaminopimelate epimerase
      Short name=DAP epimerase
    EC=5.1.1.7

Gene names

Name:	dapF
Ordered Locus Names:	BMEI0133

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP MF_00197
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP MF_00197
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diaminopimelate epimerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Diaminopimelate epimerase HAMAP MF_00197		PRO_0000149823
Sites
	Active site	76	1	By similarity
	Active site	224	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJF0-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 6C9FD0D4A9C0AA48	FASTA	303	32,368
	10 20 30 40 50 60 MATKAAFARM NGLGNQIIVA DMRGRADSIT SAAAIRLASD SETAFDQIMA IHDPRTPGTD 70 80 90 100 110 120 YYIAIINCDG TQAQACGNGT RCVVQALAAE TGRHAFTFET RAGILTATEH DDGLISVDMG 130 140 150 160 170 180 TPRFDWQDIP LAQAVADTRK IELQVDPADA PVLHSPSIAS MGNPHAVFWV DKDVWSYELD 190 200 210 220 230 240 KFGPLLENHP IFPERANISI AHVTSSDTID LRTWERGAGL TRACGSAACA AAVSAARTGR 250 260 270 280 290 300 TGRKVTVNVP GGPLLIEWRD DDHVMMTGPA EWEFSGTFDP ATGEWSRDTQ GLQGSGNADR GAA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51315.1.
PIR	AH3268.
RefSeq	NP_539051.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195845.
GenomeReviews	Gene locus BMEI0133 in contig AE008917_GR.
KEGG	bme:BMEI0133.
NMPDR	fig|224914.1.peg.133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJF0.
OMA	NVGFMQI
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0133-MON.
BRENDA	5.1.1.7. 277959.
Family and domain databases
HAMAP	MF_00197. [Tree]
InterPro	IPR001653. DAP_epimerase. IPR018510. DAP_epimerase_AS. [Graphical view]
Pfam	PF01678. DAP_epimerase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00652. DapF. 1 hit.
PROSITE	PS01326. DAP_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPF_BRUME

Accession

Primary (citable) accession number: Q8YJF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	November 24, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)