Q8YJE7 (MDH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	BMEI0137

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.
Sequence caution	The sequence AAL51319.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113443
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJE7 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: FD73DD8A3654AFB2	FASTA	320	33,666
	10 20 30 40 50 60 MARNKIALIG SGMIGGTLAH LAGLKELGDV VLFDIAEGTP QGKGLDIAES SPVDGFDAKF 70 80 90 100 110 120 TGANDYAAIE GADVVIVTAG VPRKPGMSRD DLLGINLKVM EQVGAGIKKY APEAFVICIT 130 140 150 160 170 180 NPLDAMVWAL QKFSGLPAHK VVGMAGVLDS ARFRYFLSEE FNVSVEDVTA FVLGGHGDSM 190 200 210 220 230 240 VPLARYSTVA GIPLSDLVKM GWTSQDKLDK IIQRTRDGGA EIVGLLKTGS AFYAPAASAI 250 260 270 280 290 300 QMAESYLKDK KRVLPVAAQL SGQYGVKDMY VGVPTVIGAN GVERIIEIDL DKDEKAQFDK 310 320 SVASVAGLCE ACIGIAPSLK

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51319.1. Different initiation.
PIR	AD3269.
RefSeq	NP_539055.2. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJE7.
SMR	Q8YJE7. Positions 3-311.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0137.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL51319; AAL51319; BMEI0137.
GeneID	1195849.
KEGG	bme:BMEI0137.
PATRIC	17850223. VBIBruMel92729_0143.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000213794.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	PRK06223.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_BRUME

Accession

Primary (citable) accession number: Q8YJE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program