Reviewed, UniProtKB/Swiss-Prot Q8YJD7 (TRMD_BRUME)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BMEI0149

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060343
Regions
	Region	137 – 142	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	117	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJD7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 8D38850C30D2C237	FASTA	241	26,498
	10 20 30 40 50 60 MPEKEGGRFH ASVLTLYPEM FPGPLGISLA GKALAEGKWQ LDTVQIRDFA EGRHRMVDDT 70 80 90 100 110 120 PSGGGAGMVM KADVVARALD SVDDGRPMLL MTPRGKPLTQ ERVRALADGA GAIILCGRFE 130 140 150 160 170 180 GVDERVIEGR NLEEISIGDY ILSGGETAAI VLLDAVVRLL PGVMGNRESG ETESFETGLL 190 200 210 220 230 240 EHPHYTRPQE WEGRAIPDIL TSGNHGAIDK WRLEQAERIT RERRPDLWEA YCKNRRKIGG Q

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51331.1.
PIR	AH3270.
RefSeq	NP_539067.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195861.
GenomeReviews	Gene locus BMEI0149 in contig AE008917_GR.
KEGG	bme:BMEI0149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJD7.
OMA	HRSVDDT
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0149-MON.
BRENDA	2.1.1.31. 277959.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BRUME

Accession

Primary (citable) accession number: Q8YJD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	March 1, 2002
Last modified:	November 24, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)