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Protein

DNA translocase FtsK

Gene

ftsK

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi472 – 4776ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. chromosome segregation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA translocase FtsK
Gene namesi
Name:ftsK
Ordered Locus Names:BMEI0168
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cell inner membrane By similarity; Multi-pass membrane protein By similarity
Note: Located at the septum.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5121HelicalSequence AnalysisAdd
BLAST
Transmembranei87 – 10721HelicalSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Transmembranei145 – 16521HelicalSequence AnalysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence AnalysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence AnalysisAdd
BLAST
Topological domaini247 – 817571CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 817817DNA translocase FtsKPRO_0000098243Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Forms a ring that surrounds DNA (By similarity).By similarity

Protein-protein interaction databases

STRINGi224914.BMEI0168.

Structurei

3D structure databases

ProteinModelPortaliQ8YJB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini452 – 671220FtsKPROSITE-ProRule annotationAdd
BLAST

Domaini

Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase (By similarity).By similarity

Sequence similaritiesi

Belongs to the FtsK/SpoIIIE/SftA family.Curated
Contains 1 FtsK domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000010003.
KOiK03466.
OMAiFADIVMQ.
OrthoDBiEOG6S52GD.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR025199. DUF4117.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13491. DUF4117. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YJB8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRQGYSPSYP LRDERITEDR LRFANLVRRQ VYILLGLGLL SLTAMAVGAL
60 70 80 90 100
ATWNVADPSF SHATDNPVTN ALGYPGAVFS DLAMQFFGLA SVPALLPLAV
110 120 130 140 150
WSLLLMIRGY IGRIARRSLA WVGAALLFAA IASCFAVPQS WPMPIGLGGV
160 170 180 190 200
FGDMLLRIPG FFLGGFPQGA IASAIALVLA FPALWFCFFA SGIIGRGAEA
210 220 230 240 250
VNPAMLSANR SADDEFADED ADNEAGGGFH FIGALTHLVL MTTATIRRMT
260 270 280 290 300
GLGRRRSRED DFDDMRMVRR SAETRNAPPP RARKARVEQT APSPKPGPRA
310 320 330 340 350
QREAQPSFLK DNGIFEMPSL HFLAEPKLVQ RDPALSKDAL EQNARLLAGV
360 370 380 390 400
LEDFGVRGEI INVKPGPVVT LYELEPAPGI KSSRVIGLAD DIARSMSAIA
410 420 430 440 450
ARVAVIPGRN AIGIELPNPK REMVYLREML ASRDFEQSKA KLALALGKTI
460 470 480 490 500
NGEPVIADIA KMPHVLVAGT TGSGKSVAIN TMILSLLYRM TPQECRLIMI
510 520 530 540 550
DPKMLELSVY DGIPHLLTPV VTDPKKAVVA LKWTVREMED RYRKMSKVGV
560 570 580 590 600
RNIDGFNQRV GLAQKKGEPI ARTVQTGFDR NTGEAIYETE ELDLEPMPYI
610 620 630 640 650
VVIIDEMADL MMVAGKDIEG AVQRLAQMAR AAGIHVIMAT QRPSVDVITG
660 670 680 690 700
TIKANFPTRI SFQVTSKIDS RTILGEQGAE QLLGQGDMLF MAGGGRIQRV
710 720 730 740 750
HGPFVGDDEV ERIVQHLKLQ GVPEYLDAIT EDEDDDEGGS GPAGTGNLED
760 770 780 790 800
SDDPYDQAVA VVLRDKKAST SYIQRRLGIG YNRAASIIER MEDEGIVGPA
810
NHAGKREILV PTGDDDF
Length:817
Mass (Da):88,806
Last modified:August 29, 2003 - v2
Checksum:i7F94030AACA316DF
GO

Sequence cautioni

The sequence AAL51350.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51350.1. Different initiation.
PIRiAC3273.
RefSeqiNP_539086.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL51350; AAL51350; BMEI0168.
GeneIDi1195880.
KEGGibme:BMEI0168.
PATRICi17850291. VBIBruMel92729_0177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51350.1. Different initiation.
PIRiAC3273.
RefSeqiNP_539086.1. NC_003317.1.

3D structure databases

ProteinModelPortaliQ8YJB8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BMEI0168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL51350; AAL51350; BMEI0168.
GeneIDi1195880.
KEGGibme:BMEI0168.
PATRICi17850291. VBIBruMel92729_0177.

Phylogenomic databases

HOGENOMiHOG000010003.
KOiK03466.
OMAiFADIVMQ.
OrthoDBiEOG6S52GD.

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-171-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR025199. DUF4117.
IPR002543. FtsK_dom.
IPR018541. Ftsk_gamma.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13491. DUF4117. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50901. FTSK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiFTSK_BRUME
AccessioniPrimary (citable) accession number: Q8YJB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: August 29, 2003
Last modified: January 7, 2015
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.