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Q8YJB8 (FTSK_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA translocase FtsK
Gene names
Name:ftsK
Ordered Locus Names:BMEI0168
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length817 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif By similarity.

Subunit structure

Homohexamer. Forms a ring that surrounds DNA By similarity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity. Note: Located at the septum By similarity.

Domain

Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase By similarity.

Sequence similarities

Belongs to the FtsK/SpoIIIE/SftA family.

Contains 1 FtsK domain.

Sequence caution

The sequence AAL51350.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 817817DNA translocase FtsK
PRO_0000098243

Regions

Transmembrane31 – 5121Helical; Potential
Transmembrane87 – 10721Helical; Potential
Transmembrane118 – 13821Helical; Potential
Transmembrane145 – 16521Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane226 – 24621Helical; Potential
Topological domain247 – 817571Cytoplasmic Potential
Domain452 – 671220FtsK
Nucleotide binding472 – 4776ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YJB8 [UniParc].

Last modified August 29, 2003. Version 2.
Checksum: 7F94030AACA316DF

FASTA81788,806
        10         20         30         40         50         60 
MRQGYSPSYP LRDERITEDR LRFANLVRRQ VYILLGLGLL SLTAMAVGAL ATWNVADPSF 

        70         80         90        100        110        120 
SHATDNPVTN ALGYPGAVFS DLAMQFFGLA SVPALLPLAV WSLLLMIRGY IGRIARRSLA 

       130        140        150        160        170        180 
WVGAALLFAA IASCFAVPQS WPMPIGLGGV FGDMLLRIPG FFLGGFPQGA IASAIALVLA 

       190        200        210        220        230        240 
FPALWFCFFA SGIIGRGAEA VNPAMLSANR SADDEFADED ADNEAGGGFH FIGALTHLVL 

       250        260        270        280        290        300 
MTTATIRRMT GLGRRRSRED DFDDMRMVRR SAETRNAPPP RARKARVEQT APSPKPGPRA 

       310        320        330        340        350        360 
QREAQPSFLK DNGIFEMPSL HFLAEPKLVQ RDPALSKDAL EQNARLLAGV LEDFGVRGEI 

       370        380        390        400        410        420 
INVKPGPVVT LYELEPAPGI KSSRVIGLAD DIARSMSAIA ARVAVIPGRN AIGIELPNPK 

       430        440        450        460        470        480 
REMVYLREML ASRDFEQSKA KLALALGKTI NGEPVIADIA KMPHVLVAGT TGSGKSVAIN 

       490        500        510        520        530        540 
TMILSLLYRM TPQECRLIMI DPKMLELSVY DGIPHLLTPV VTDPKKAVVA LKWTVREMED 

       550        560        570        580        590        600 
RYRKMSKVGV RNIDGFNQRV GLAQKKGEPI ARTVQTGFDR NTGEAIYETE ELDLEPMPYI 

       610        620        630        640        650        660 
VVIIDEMADL MMVAGKDIEG AVQRLAQMAR AAGIHVIMAT QRPSVDVITG TIKANFPTRI 

       670        680        690        700        710        720 
SFQVTSKIDS RTILGEQGAE QLLGQGDMLF MAGGGRIQRV HGPFVGDDEV ERIVQHLKLQ 

       730        740        750        760        770        780 
GVPEYLDAIT EDEDDDEGGS GPAGTGNLED SDDPYDQAVA VVLRDKKAST SYIQRRLGIG 

       790        800        810 
YNRAASIIER MEDEGIVGPA NHAGKREILV PTGDDDF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL51350.1. Different initiation.
PIRAC3273.
RefSeqNP_539086.1. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YJB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0168.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL51350; AAL51350; BMEI0168.
GeneID1195880.
KEGGbme:BMEI0168.
PATRIC17850291. VBIBruMel92729_0177.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000010003.
KOK03466.
OMASEDPYDQ.
OrthoDBEOG6S52GD.
ProtClustDBCLSK898034.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-171-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR002543. Cell_div_FtsK/SpoIIIE.
IPR018541. DNA_translocase_Ftsk_gamma.
IPR025199. DUF4117.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF13491. DUF4117. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50901. FTSK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTSK_BRUME
AccessionPrimary (citable) accession number: Q8YJB8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: August 29, 2003
Last modified: April 16, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names