Q8YJB0 (HEM3_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	BMEI0176

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. HAMAP MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity. HAMAP MF_00260
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP MF_00260
Subunit structure	Monomer By similarity. HAMAP MF_00260
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethan group. HAMAP MF_00260
Sequence similarities	Belongs to the HMBS family.
Sequence caution	The sequence AAL51358.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-pyrromethane cofactor linkage Inferred from electronic annotation. Source: InterPro porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Porphobilinogen deaminase HAMAP MF_00260		PRO_0000142914
Amino acid modifications
	Modified residue	249	1	S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJB0 [UniParc]. Last modified October 10, 2002. Version 2. Checksum: D4F34A01D72322B6	FASTA	314	33,838
	10 20 30 40 50 60 MQTASFKNGT LKIGTRGSKL ALAQAYLTRR LLQEAHGLPE DAIEILPMST AGDRIQDRPL 70 80 90 100 110 120 SEVGGKGLFT EEIEQALKDG RIDIAVHSTK DMPTALPEGL HLSVFLERED PRDAFIGRSA 130 140 150 160 170 180 RRFMDLPQGA TVGSSSLRRQ ALIRRLRPDI EVVMYRGNVD TRLRKLDAGE VDGTFLACAG 190 200 210 220 230 240 LRRLGLADVI TDVLDPSVFP PAPGQGAIGI ESRIGDERID VLLAPLAHRE TQIALACERA 250 260 270 280 290 300 FLGALDGSCR TPIAGLATVE GDRLSFRGMI LTPDGRQAHE VTAEGVVSDA AALGTDAANR 310 VRAMAGPHFF DGWQ

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51358.1. Different initiation.
PIR	AC3274.
RefSeq	NP_539094.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJB0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195888.
GenomeReviews	Gene locus BMEI0176 in contig AE008917_GR.
KEGG	bme:BMEI0176.
PATRIC	17850309. VBIBruMel92729_0186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG624842.
OMA	FKDVRGN.
PhylomeDB	Q8YJB0.
ProtClustDB	PRK00072.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0176-MONOMER.
Family and domain databases
HAMAP	MF_00260. Porphobil_deam. [Tree]
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022419. Porphobilin_deaminase_cofac_BS. IPR022417. Porphobilin_deaminase_N. IPR022418. Porphobilinogen_deaminase_C. [Graphical view]
Gene3D	G3DSA:3.30.160.40. Porphobil_deam. 1 hit.
KO	K01749.
PANTHER	PTHR11557. Porphobil_deam. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. PF03900. Porphobil_deamC. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
SUPFAM	SSF54782. Porphobil_deam. 1 hit.
TIGRFAMs	TIGR00212. HemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_BRUME

Accession

Primary (citable) accession number: Q8YJB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program