Reviewed, UniProtKB/Swiss-Prot Q8YJB0 (HEM3_BRUME)

Names and origin

Protein names

Recommended name:
    Porphobilinogen deaminase
      Short name=PBG
    EC=2.5.1.61
Alternative name(s):
    Hydroxymethylbilane synthase
      Short name=HMBS
    Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	BMEI0176

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. HAMAP MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP MF_00260
Subunit structure	Monomer By similarity.
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethan group. HAMAP MF_00260
Sequence similarities	Belongs to the HMBS family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Porphobilinogen deaminase HAMAP MF_00260		PRO_0000142914
Sites
	Binding site	249	1	Pyrromethane cofactor (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJB0-1 [UniParc]. Last modified October 10, 2002. Version 2. Checksum: D4F34A01D72322B6	FASTA	314	33,838
	10 20 30 40 50 60 MQTASFKNGT LKIGTRGSKL ALAQAYLTRR LLQEAHGLPE DAIEILPMST AGDRIQDRPL 70 80 90 100 110 120 SEVGGKGLFT EEIEQALKDG RIDIAVHSTK DMPTALPEGL HLSVFLERED PRDAFIGRSA 130 140 150 160 170 180 RRFMDLPQGA TVGSSSLRRQ ALIRRLRPDI EVVMYRGNVD TRLRKLDAGE VDGTFLACAG 190 200 210 220 230 240 LRRLGLADVI TDVLDPSVFP PAPGQGAIGI ESRIGDERID VLLAPLAHRE TQIALACERA 250 260 270 280 290 300 FLGALDGSCR TPIAGLATVE GDRLSFRGMI LTPDGRQAHE VTAEGVVSDA AALGTDAANR 310 VRAMAGPHFF DGWQ

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51358.1. Different initiation.
PIR	AC3274.
RefSeq	NP_539094.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195888.
GenomeReviews	Gene locus BMEI0176 in contig AE008917_GR.
KEGG	bme:BMEI0176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJB0.
OMA	AKIGDKG
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0176-MON.
BRENDA	2.5.1.61. 277959.
Family and domain databases
HAMAP	MF_00260. [Tree]
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. [Graphical view]
Gene3D	G3DSA:3.30.160.40. Porphobil_deam. 1 hit.
PANTHER	PTHR11557. Porphobil_deam. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. PF03900. Porphobil_deamC. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
TIGRFAMs	TIGR00212. hemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_BRUME

Accession

Primary (citable) accession number: Q8YJB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	November 24, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)