Q8YJ98 (UBIG_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-demethylubiquinone-9 3-methyltransferase
EC=2.1.1.64

Alternative name(s):
2-polyprenyl-6-hydroxyphenyl methylase
EC=2.1.1.222
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase
Short name=DHHB methyltransferase

Gene names

Name:	ubiG
Ordered Locus Names:	BMEI0188

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-specific O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway By similarity.
Catalytic activity	S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n. HAMAP MF_00472 S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol.
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP MF_00472
Sequence similarities	Belongs to the methyltransferase superfamily. UbiG/COQ3 family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from electronic annotation. Source: InterPro 3-demethylubiquinone-9 3-O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	3-demethylubiquinone-9 3-methyltransferase HAMAP MF_00472		PRO_0000193372

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ98 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E413433494D7B9B7	FASTA	248	27,516
	10 20 30 40 50 60 MTETARTTID ASEIEHFSRI AAQWWDPQGK FRPLHKFNPT RLAYIKEKVC AKFNRDPNAP 70 80 90 100 110 120 RPLEGLRFLD IGCGGGLLCE PMARLGATVI GADASATNIE VAKIHAAQSS LDIDYRATTT 130 140 150 160 170 180 EALADAGEKF DVVLNMEVVE HVSDVDLFMS ATSAMVKPGG LMFVATINRT LKAYGLAIIG 190 200 210 220 230 240 AEYVLRWLPR GTHQYEKLVR PEELEAAFSK ADLRLIDKLG VTYNPLADSW NRSRDMDVNY MVLAERPA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51370.1.
PIR	AG3275.
RefSeq	NP_539106.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJ98.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195900.
GenomeReviews	Gene locus BMEI0188 in contig AE008917_GR.
KEGG	bme:BMEI0188.
NMPDR	fig|224914.1.peg.188.
PATRIC	17795297. VBIBruMel146950_0012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG738842.
OMA	KFITPDE.
PhylomeDB	Q8YJ98.
ProtClustDB	PRK05134.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0188-MONOMER.
Family and domain databases
HAMAP	MF_00472. UbiG. [Tree]
InterPro	IPR013216. Methyltransf_11. IPR010233. UbiG_MeTrfase. [Graphical view]
KO	K00568.
PANTHER	PTHR23134. PTHR23134. 1 hit.
Pfam	PF08241. Methyltransf_11. 1 hit. [Graphical view]
TIGRFAMs	TIGR01983. UbiG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UBIG_BRUME

Accession

Primary (citable) accession number: Q8YJ98

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program