Reviewed, UniProtKB/Swiss-Prot Q8YJ95 (RF1_BRUME)

Names and origin

Protein names

Recommended name:
    Peptide chain release factor 1
      Short name=RF-1

Gene names

Name:	prfA
Ordered Locus Names:	BMEI0191

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translational termination Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Peptide chain release factor 1 HAMAP MF_00093		PRO_0000177644

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ95-1 [UniParc]. Last modified April 3, 2002. Version 2. Checksum: 9B447F031AD72D84	FASTA	359	39,909
	10 20 30 40 50 60 MIALPQDRMD QLLKRFSMIE SQMANNPDSD TYVKLASEYS ELQDVVGKIR ELSDARMEAS 70 80 90 100 110 120 DLAAMRDDAS TDAEMRALAV EELPEVEKRI AVLEQDVQIL LLPKDAADDK NAILEIRAGT 130 140 150 160 170 180 GGLEAALFAG DLFRMYERYA AEKGWRVELV SASEGDAGGY KEIIATVSGK GVFSKLKFES 190 200 210 220 230 240 GVHRVQRVPE TEAGGRIHTS AATVAVLPEA EDIDIEIRNE DIRIDTMRAS GAGGQHVNTT 250 260 270 280 290 300 DSAVRITHIP TGIMVVQAEK SQHQNRARAM QILRARLYDM ERQKAESERS QARRSQVGSG 310 320 330 340 350 DRSERIRTYN FPQGRVTDHR INLTLYKLDR VMEGELDELV DALISDHQTA LLAELGEQP

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51373.1. Different initiation.
PIR	AB3276.
RefSeq	NP_539109.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195903.
GenomeReviews	Gene locus BMEI0191 in contig AE008917_GR.
KEGG	bme:BMEI0191.
NMPDR	fig|224914.1.peg.191.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJ95.
OMA	EAGGYKE
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0191-MON.
Family and domain databases
HAMAP	MF_00093. [Tree]
InterPro	IPR005139. PCRF. IPR000352. Pep_rel_factor_I. IPR004373. PrfA. [Graphical view]
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00019. prfA. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF1_BRUME

Accession

Primary (citable) accession number: Q8YJ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	April 3, 2002
Last modified:	November 24, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)