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Q8YJ95 (RF1_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide chain release factor 1

Short name=RF-1
Gene names
Name:prfA
Ordered Locus Names:BMEI0191
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP-Rule MF_00093

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00093.

Post-translational modification

Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP-Rule MF_00093

Sequence similarities

Belongs to the prokaryotic/mitochondrial release factor family.

Sequence caution

The sequence AAL51373.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   PTMMethylation
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontranslation release factor activity, codon specific

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Peptide chain release factor 1 HAMAP-Rule MF_00093
PRO_0000177644

Amino acid modifications

Modified residue2351N5-methylglutamine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8YJ95 [UniParc].

Last modified April 3, 2002. Version 2.
Checksum: 9B447F031AD72D84

FASTA35939,909
        10         20         30         40         50         60 
MIALPQDRMD QLLKRFSMIE SQMANNPDSD TYVKLASEYS ELQDVVGKIR ELSDARMEAS 

        70         80         90        100        110        120 
DLAAMRDDAS TDAEMRALAV EELPEVEKRI AVLEQDVQIL LLPKDAADDK NAILEIRAGT 

       130        140        150        160        170        180 
GGLEAALFAG DLFRMYERYA AEKGWRVELV SASEGDAGGY KEIIATVSGK GVFSKLKFES 

       190        200        210        220        230        240 
GVHRVQRVPE TEAGGRIHTS AATVAVLPEA EDIDIEIRNE DIRIDTMRAS GAGGQHVNTT 

       250        260        270        280        290        300 
DSAVRITHIP TGIMVVQAEK SQHQNRARAM QILRARLYDM ERQKAESERS QARRSQVGSG 

       310        320        330        340        350 
DRSERIRTYN FPQGRVTDHR INLTLYKLDR VMEGELDELV DALISDHQTA LLAELGEQP 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL51373.1. Different initiation.
PIRAB3276.
RefSeqNP_539109.2. NC_003317.1.

3D structure databases

ProteinModelPortalQ8YJ95.
SMRQ8YJ95. Positions 68-355.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0191.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL51373; AAL51373; BMEI0191.
GeneID1195903.
KEGGbme:BMEI0191.
PATRIC17850339. VBIBruMel92729_0201.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000074815.
KOK02835.
OMANDINDRE.
OrthoDBEOG6TN48J.

Family and domain databases

Gene3D3.30.160.20. 1 hit.
HAMAPMF_00093. Rel_fac_1.
InterProIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsTIGR00019. prfA. 1 hit.
PROSITEPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRF1_BRUME
AccessionPrimary (citable) accession number: Q8YJ95
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 3, 2002
Last modified: May 14, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names