Q8YJ95 (RF1_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1
Short name=RF-1

Gene names

Name:	prfA
Ordered Locus Names:	BMEI0191

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP MF_00093
Subcellular location	Cytoplasm HAMAP MF_00093.
Post-translational modification	Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP MF_00093
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.
Sequence caution	The sequence AAL51373.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation release factor activity, codon specific Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Peptide chain release factor 1 HAMAP MF_00093		PRO_0000177644
Amino acid modifications
	Modified residue	235	1	N5-methylglutamine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ95 [UniParc]. Last modified April 3, 2002. Version 2. Checksum: 9B447F031AD72D84	FASTA	359	39,909
	10 20 30 40 50 60 MIALPQDRMD QLLKRFSMIE SQMANNPDSD TYVKLASEYS ELQDVVGKIR ELSDARMEAS 70 80 90 100 110 120 DLAAMRDDAS TDAEMRALAV EELPEVEKRI AVLEQDVQIL LLPKDAADDK NAILEIRAGT 130 140 150 160 170 180 GGLEAALFAG DLFRMYERYA AEKGWRVELV SASEGDAGGY KEIIATVSGK GVFSKLKFES 190 200 210 220 230 240 GVHRVQRVPE TEAGGRIHTS AATVAVLPEA EDIDIEIRNE DIRIDTMRAS GAGGQHVNTT 250 260 270 280 290 300 DSAVRITHIP TGIMVVQAEK SQHQNRARAM QILRARLYDM ERQKAESERS QARRSQVGSG 310 320 330 340 350 DRSERIRTYN FPQGRVTDHR INLTLYKLDR VMEGELDELV DALISDHQTA LLAELGEQP

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51373.1. Different initiation.
PIR	AB3276.
RefSeq	NP_539109.2. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YJ95.
SMR	Q8YJ95. Positions 68-355.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195903.
GenomeReviews	Gene locus BMEI0191 in contig AE008917_GR.
KEGG	bme:BMEI0191.
NMPDR	fig|224914.1.peg.191.
PATRIC	17850339. VBIBruMel92729_0201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG629764.
OMA	GEAGGYK.
PhylomeDB	Q8YJ95.
ProtClustDB	PRK00591.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0191-MONOMER.
Family and domain databases
HAMAP	MF_00093. Rel_fac_1. [Tree]
InterPro	IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. IPR004373. PrfA. [Graphical view]
KO	K02835.
PANTHER	PTHR11075:SF9. PrfA. 1 hit.
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00019. PrfA. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF1_BRUME

Accession

Primary (citable) accession number: Q8YJ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	April 3, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program