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Protein

Peptide chain release factor 1

Gene

prfA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 1UniRule annotation
Short name:
RF-1UniRule annotation
Gene namesi
Name:prfAUniRule annotation
Ordered Locus Names:BMEI0191
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Peptide chain release factor 1PRO_0000177644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei235 – 2351N5-methylglutamineUniRule annotation

Post-translational modificationi

Methylated by PrmC. Methylation increases the termination efficiency of RF1.UniRule annotation

Keywords - PTMi

Methylation

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_0006.

Structurei

3D structure databases

ProteinModelPortaliQ8YJ95.
SMRiQ8YJ95. Positions 68-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prokaryotic/mitochondrial release factor family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000074815.
KOiK02835.
OMAiVAMQNEK.
OrthoDBiEOG6TN48J.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YJ95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALPQDRMD QLLKRFSMIE SQMANNPDSD TYVKLASEYS ELQDVVGKIR
60 70 80 90 100
ELSDARMEAS DLAAMRDDAS TDAEMRALAV EELPEVEKRI AVLEQDVQIL
110 120 130 140 150
LLPKDAADDK NAILEIRAGT GGLEAALFAG DLFRMYERYA AEKGWRVELV
160 170 180 190 200
SASEGDAGGY KEIIATVSGK GVFSKLKFES GVHRVQRVPE TEAGGRIHTS
210 220 230 240 250
AATVAVLPEA EDIDIEIRNE DIRIDTMRAS GAGGQHVNTT DSAVRITHIP
260 270 280 290 300
TGIMVVQAEK SQHQNRARAM QILRARLYDM ERQKAESERS QARRSQVGSG
310 320 330 340 350
DRSERIRTYN FPQGRVTDHR INLTLYKLDR VMEGELDELV DALISDHQTA

LLAELGEQP
Length:359
Mass (Da):39,909
Last modified:April 3, 2002 - v2
Checksum:i9B447F031AD72D84
GO

Sequence cautioni

The sequence AAL51373.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51373.1. Different initiation.
PIRiAB3276.
RefSeqiWP_004686702.1. NZ_GG703778.1.

Genome annotation databases

EnsemblBacteriaiAAL51373; AAL51373; BMEI0191.
KEGGibme:BMEI0191.
PATRICi17850339. VBIBruMel92729_0201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51373.1. Different initiation.
PIRiAB3276.
RefSeqiWP_004686702.1. NZ_GG703778.1.

3D structure databases

ProteinModelPortaliQ8YJ95.
SMRiQ8YJ95. Positions 68-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_0006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL51373; AAL51373; BMEI0191.
KEGGibme:BMEI0191.
PATRICi17850339. VBIBruMel92729_0201.

Phylogenomic databases

HOGENOMiHOG000074815.
KOiK02835.
OMAiVAMQNEK.
OrthoDBiEOG6TN48J.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiRF1_BRUME
AccessioniPrimary (citable) accession number: Q8YJ95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 3, 2002
Last modified: July 22, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.