Q8YJ80 (OBG_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase obg

Alternative name(s):
GTP-binding protein obg

Gene names

Name:	obg
Ordered Locus Names:	BMEI0206

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate By similarity. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control Potential. HAMAP MF_01454
Cofactor	Magnesium Potential.
Subunit structure	Monomer By similarity. HAMAP MF_01454
Subcellular location	Cytoplasm By similarity HAMAP MF_01454.
Sequence similarities	Belongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.
Sequence caution	The sequence AAL51388.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	GTPase obg HAMAP MF_01454		PRO_0000385769
Regions
	Domain	171 – 278	108	G
	Nucleotide binding	166 – 173	8	GTP By similarity
	Nucleotide binding	212 – 216	5	GTP By similarity
	Nucleotide binding	279 – 282	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ80 [UniParc]. Last modified October 13, 2009. Version 2. Checksum: 7C440746D70AB29B	FASTA	341	36,789
	10 20 30 40 50 60 MKFLDQAKIY IRSGNGGAGA VSFRREKFLE FGGPDGGDGG RGGDVWVEAV DGLNTLIDYR 70 80 90 100 110 120 YQQHFKAKTG MHGMGRNMTG GKGDDVVLRV PVGTQIFEED NETLICDITE VGQRYRLAKG 130 140 150 160 170 180 GNGGFGNLHF TTSTNRAPRR ANPGQEGIER TIWLRLKLIA DAGLVGLPNA GKSTFLASVT 190 200 210 220 230 240 AAKPKIADYP FTTLHPNLGV ARIDGREFVI ADIPGLIEGA SEGVGLGDRF LGHVERTRVL 250 260 270 280 290 300 LHLVSAQEED VAKAYQVIRG ELEAYEHGLA DKPEIVALSQ VDTLDPETRK AKVKALKKAC 310 320 330 340 GCEPLLLSAV SHEGLNDTLR QLARIIDLSR AEEAGTAQAE E

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51388.1. Different initiation.
PIR	AI3277.
RefSeq	NP_539124.2. NC_003317.1.
3D structure databases
HSSP	HSSP built from PDB template 1LNZ based on UniProtKB P20964.
ProteinModelPortal	Q8YJ80.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195918.
GenomeReviews	Gene locus BMEI0206 in contig AE008917_GR.
KEGG	bme:BMEI0206.
PATRIC	17850398. VBIBruMel92729_0222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG716038.
ProtClustDB	PRK12299.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0206-MONOMER.
Family and domain databases
HAMAP	MF_01454. GTPase_Obg. [Tree]
InterPro	IPR014100. GTP-bd_Obg/CgtA. IPR006074. GTP1-OBG_CS. IPR006169. GTP1_OBG_dom. IPR006073. GTP_binding_domain. [Graphical view]
Gene3D	G3DSA:2.70.210.12. GTP1_OBG_sub. 1 hit.
KO	K03979.
PANTHER	PTHR11702:SF3. GTP_bd_Obg/CgtA. 1 hit.
Pfam	PF01018. GTP1_OBG. 1 hit. PF01926. MMR_HSR1. 1 hit. [Graphical view]
PIRSF	PIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
PRINTS	PR00326. GTP1OBG.
SUPFAM	SSF82051. GTP1_OBG_sub. 1 hit.
TIGRFAMs	TIGR02729. Obg_CgtA. 1 hit.
PROSITE	PS00905. GTP1_OBG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OBG_BRUME

Accession

Primary (citable) accession number: Q8YJ80

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 13, 2009
Last sequence update:	October 13, 2009
Last modified:	January 25, 2012
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program