Reviewed, UniProtKB/Swiss-Prot Q8YJ78 (PROA_BRUME)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BMEI0208

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase		PRO_0000189706

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ78-1 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 89798D02ACE81350	FASTA	421	44,094
	10 20 30 40 50 60 MTKDIAQVMA EVGRKAKAAA APLSIATNEQ KNKALNAAAD AILEARADIL EANRLDLANA 70 80 90 100 110 120 EKNGMAASFV DRLTLNEARI DAIAEGIRAI ATLPDPVGEV IAEWDRPNGL HIERVRTPLG 130 140 150 160 170 180 VIGVIYESRP NVTADAGALC LKAGNAVILR GGSDSAHSSA AIYKALVKGL EAANLPADAI 190 200 210 220 230 240 QIVPVTDRAA VGEMLKGLGG AIDVIVPRGG KSLVARVQSE ARVPVFAHLE GICHLYIDKS 250 260 270 280 290 300 ADLDMARRIA LDAKMRRTGI CGAAETLLVD RAVASTHLAP ILGDLAAGGC EIRGSAEVLA 310 320 330 340 350 360 LYPAAKPATE EDWSTEYLDA IISVALVDGI SGAIDHINRY SSHHTEAIVA EDAQTVARFF 370 380 390 400 410 420 NEIDSAILLH NASTQFADGG EFGMGAEIGI ATGKMHARGP VGVEQLTSFK YRVRGSGQVR G

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009463 Genomic DNA. Translation: AAL51390.1. Different initiation.
PIR	AC3278.
RefSeq	NP_539126.1.
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
GeneID	1195920.
GenomeReviews	Gene locus BMEI0208 in contig AE008917_GR.
KEGG	bme:BMEI0208.
NMPDR	fig|224914.1.peg.208.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YJ78.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0208-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BRUME

Accession

Primary (citable) accession number: Q8YJ78

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	November 7, 2003
Last modified:	November 25, 2008
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)