Q8YJ78 (PROA_BRUME)
Reviewed,
UniProtKB/Swiss-Prot
Last modified
August 10, 2010.
Version 63.
History...
Names and origin
| Protein names | Recommended name: Gamma-glutamyl phosphate reductase Short name=GPR EC=1.2.1.41 Alternative name(s): Glutamate-5-semialdehyde dehydrogenase Glutamyl-gamma-semialdehyde dehydrogenase Short name=GSA dehydrogenase | ||||
| Gene names |
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| Organism | Brucella melitensis [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 29459 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 421 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412 |
| Catalytic activity | L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412 |
| Pathway | Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00412. |
| Sequence similarities | Belongs to the gamma-glutamyl phosphate reductase family. |
| Sequence caution | The sequence AAL51390.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Proline biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 421 | 421 | Gamma-glutamyl phosphate reductase HAMAP MF_00412 | PRO_0000189706 | |||
Sequences
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References
| [1] | "The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. Overbeek R.Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE008917 Genomic DNA. Translation: AAL51390.1. Different initiation. |
| PIR | AC3278. |
| RefSeq | NP_539126.1. |
3D structure databases | |
| ProteinModelPortal | Q8YJ78. |
| SMR | Q8YJ78. Positions 13-421. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1195920. |
| GenomeReviews | Gene locus BMEI0208 in contig AE008917_GR. |
| KEGG | bme:BMEI0208. |
| NMPDR | fig|224914.1.peg.208. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG318080. |
| OMA | ITHINRY. |
| PhylomeDB | Q8YJ78. |
| ProtClustDB | PRK00197. |
Enzyme and pathway databases | |
| BioCyc | BMEL224914:BMEI0208-MONOMER. |
| BRENDA | 1.2.1.41. 277959. |
Family and domain databases | |
| HAMAP | MF_00412. ProA. [Tree] |
| InterPro | IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view] |
| Gene3D | G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| PANTHER | PTHR11063:SF1. GSA_DH. 1 hit. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| PIRSF | PIRSF000151. GPR. 1 hit. |
| SUPFAM | SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit. |
| TIGRFAMs | TIGR00407. proA. 1 hit. |
| PROSITE | PS01223. PROA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PROA_BRUME | ||||||||
| Accession | Primary (citable) accession number: Q8YJ78 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Brucella melitensis Brucella melitensis (strain 16M): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


