Q8YJ78 (PROA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BMEI0208

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.
Sequence caution	The sequence AAL51390.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189706

Sequences

	Sequence		Length	Mass (Da)
	Q8YJ78-1 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 89798D02ACE81350	FASTA	421	44,094
	10 20 30 40 50 60 MTKDIAQVMA EVGRKAKAAA APLSIATNEQ KNKALNAAAD AILEARADIL EANRLDLANA 70 80 90 100 110 120 EKNGMAASFV DRLTLNEARI DAIAEGIRAI ATLPDPVGEV IAEWDRPNGL HIERVRTPLG 130 140 150 160 170 180 VIGVIYESRP NVTADAGALC LKAGNAVILR GGSDSAHSSA AIYKALVKGL EAANLPADAI 190 200 210 220 230 240 QIVPVTDRAA VGEMLKGLGG AIDVIVPRGG KSLVARVQSE ARVPVFAHLE GICHLYIDKS 250 260 270 280 290 300 ADLDMARRIA LDAKMRRTGI CGAAETLLVD RAVASTHLAP ILGDLAAGGC EIRGSAEVLA 310 320 330 340 350 360 LYPAAKPATE EDWSTEYLDA IISVALVDGI SGAIDHINRY SSHHTEAIVA EDAQTVARFF 370 380 390 400 410 420 NEIDSAILLH NASTQFADGG EFGMGAEIGI ATGKMHARGP VGVEQLTSFK YRVRGSGQVR G

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51390.1. Different initiation.
PIR	AC3278.
RefSeq	NP_539126.1.
3D structure databases
ProteinModelPortal	Q8YJ78.
SMR	Q8YJ78. Positions 13-421.
ModBase	Search...
Genome annotation databases
GeneID	1195920.
GenomeReviews	Gene locus BMEI0208 in contig AE008917_GR.
KEGG	bme:BMEI0208.
NMPDR	fig|224914.1.peg.208.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	ITHINRY.
PhylomeDB	Q8YJ78.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0208-MONOMER.
BRENDA	1.2.1.41. 277959.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BRUME

Accession

Primary (citable) accession number: Q8YJ78

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	November 7, 2003
Last modified:	August 10, 2010
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)